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significance of small size of bacteria
Bright field microscopy
total magnifying power
resolution
How can we create contrast (in GENERAL) in bright-field microscopy?
gram stain
disadvantages of gram staining
How can we create contrast optically?
Transmission Electron Microscopy (TEM)
Visualization: Immunofluorescence Microscopy
Pros/Cons of Immunofluorescence
Visualization: Protein fusions to Green Fluorescent Protein (GFP)
Pros/Cons of GFP visualization
Steps AFTER GFP visualization
How do we know an IF or GFP experiment is showing us the REAL location of the protein?
Immunofluorescence vs GFP
Direct Microscopic Count
Viable cell count (including disadvantages)
Turbidity
Pros/Cons of OD count
