Direct cell counts can be divided into 2. Total count and viable count
Total count measure the total number of dead cells
A bacillus bacteria is spherical
A cocci bacteria is round
Before gram staining bacteria is colourless
Gram negative bacteria is susceptible to antibiotics and antibacterial agents
The gram positive cell wall is more complex than gram negative's cell wall
Aseptic refers to a procedure performed under sterile conditions
Gram negative has a thin layer of peptidoglycan
A pathogen is a disease causing micro-organism
A colony of bacteria arises from more than one bacteria
An air filter bubbles air through the fermenter
A secondary metabolite is produced after an exponential growth of fungus is complete
Other methods of measuring bacteria growth includes haemocytometer and turbidmetry
Facultative anaerobes grow better in presence of oxygen however they can also grow in absence of oxyegen
Optimum temperature for mammalian pathogens is 25 degrees celsius.
Radiation such as gamma rays is used to sterile plastic equipment
Glass cannot be sterilised in an autoclave
Gram negative bacteria is a purple colour after staining because it has a thick layer of peptidoglycan
After sterilising the work surface, the cap of the culture bottle can be placed down
Seal petri dish and lid all the way around
Sterilise all equipment in boiling water for at least 15 minutes before use.
After innoculation leave the lid of the petri dish for a few minutes
