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Chemiluminescence
Assay
Analyte
Assay Buffer
Multiplex
Pos Control
Neg Control
Signal to Background Ratio
Assay Format
% Inhibition
Assay Design
Assay Development
Assay Validation
Accuracy
Precision
LOD
LOQ
Specificity
Linearity
Range
Ruggedness
Robustness
Direct Assay
Competitive Assay
Absorption
Absorbance
Transmittance
Fluorescence Quenching
Self quenching
Quencher
Quantum Yield
Choices for detection methods
Optimization Parameters for ELISA
Assessing an assay
Parameters essential for immunoassay validation
Advantages/Disadvantages of ELISA
Epitope
Two types of Epitopes
Monoclonal Antibodies
Polyclonal Antibodies
Details about Polyclonal
Details about Monoclonal
Different ELISA formats
Direct vs Indirect ELISA
Parameters to optimize a sandwich ELISA
Methods of coating wells with capture antibody or antigen
Protein Tags
Steps for indirect sandwich ELISA
Choices of detection method for ELISA
2 Most common types of enzymes used for ELISA
Mechanism of fluorescence
Photobleaching
Fluorescence detection
Monochromator - fluorescence
Filter-based - flourscence
environmental effects on fluorescence
Background signals that limit fluorescence sensitivity
Importance of reading Absorbance assay at max wavelength
Spectrophotometric assay
Flourescence
Coupled enzyme assays
Malachite green detection of phosphate
