Microscope techniques:

· When transporting , carry with both hand one on the arm of the microscope, one under the base
· Use a lens paper to clean all the lenses and the condenser.
· Raise the condenser to the highest position and open iris diaphragm.
· Turn the microscope lamp on and adjust to maximum intensity.
· Move the scanning (4x) or low power (10x) objective lens into place.
· Place the slide on stage center specimen over opening
· Adjust iris diaphragm to yield optimum illumination
· To focus image. First coarse to oil immersion lens; place drop of immersion oil on specimen. Submerge oil objective lens into oil, use fine focus to view image.
· Objective lenses are par focal
· When storing lower the stage, remove slide; clean lens paper, warp cord around above base of microscope.
· Return to cabinet.

Calculating Magnification:

Total magnification= objective lens magnification ×Ocular lens mag.

Resolution: the eye limits the size of the object visible to the unaided eye.

Immersion Technique:

· Adding oil has the same refractive index as glass, less refracted, more enters lens.

	Erstellt von elsiemm108 vor mehr als 10 Jahre

Notiz am Microlab review, erstellt von elsiemm108 am 30/11/2013.