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What was the goal of lab 3?
What was the goal plasmid to make during this lab? Indicate the important features of this particular plasmid and the function of each feature.
What is the role of DNA Ligase in replication? What about in gene cloning?
Why did we need to inactivate BamHI and HindIII before beginning this lab?
What was in the LIG tube? What happened in this tube?
What properties of the DNA restriction fragments allow for ligation to occur?
Could two rfp-pBAD fragments form during ligation? Why or why not?
