Creating an E.Coli Library

1

Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up

1

What does N stand for?

1

What are the meanings of i and g?

1

The larger the genome, the larger the library required to replicate it

1

When is cDNA used?

1

How is cDNA made?

1

Less than 1% of the genome is mRNA, but it's exclusively expressible genes

1

What is required to make the first RNA-DNA hybrid?

1

What are the two methods of degrading the RNA part?

1

Which method was used to get the following DNA strand?

1

What is required to get from the former to the latter?

1

What method was used to degrade the RNA into the following?

1

What is needed to get from the former to the latter?

1

When creating cDNA, a little information is always lost from the 3' end

1

How do we prevent this loss of information?

1

Then in second strand synthesis add ...

1

Enriching desired DNA means creating/finding more of it

1

Selective Hybridisation removes cDNA's common to more than one tissue

1

Tissue Specificity is useful if the gene is only expressed in a certain tissue

1

What is Normalisation?

1

How does Normalisation work?

Year 2 Quiz am Creating an E.Coli Library, erstellt von gina_evans0312 am 29/12/2013.

Creating an E.Coli Library

Electroporations- passing electricity through a mix of DNA and bacteria causes DNA to be taken up

What does N stand for?

What are the meanings of i and g?

The larger the genome, the larger the library required to replicate it

When is cDNA used?

How is cDNA made?

Less than 1% of the genome is mRNA, but it's exclusively expressible genes

What is required to make the first RNA-DNA hybrid?

What are the two methods of degrading the RNA part?

Which method was used to get the following DNA strand?

What is required to get from the former to the latter?

What method was used to degrade the RNA into the following?

What is needed to get from the former to the latter?

When creating cDNA, a little information is always lost from the 3' end

How do we prevent this loss of information?

Then in second strand synthesis add ...

Enriching desired DNA means creating/finding more of it

Selective Hybridisation removes cDNA's common to more than one tissue

Tissue Specificity is useful if the gene is only expressed in a certain tissue

What is Normalisation?