Which antibody was being investigated in this lab?

Which antigen was used to coat the plate?

Which enzyme-linked antibody was used to bind to any anti-HIV IgG? Which enzyme was attached?

Which chromogenic substrate was used?

What is a chromogen?

What type of technique is an ELISA?

What type of dilutions were performed on the serum samples?

What is the importance of washing and blotting in an ELISA?

What was the point of the positive control wells?

What was the point of the negative control wells?

What is the limit of detection?