BCM-FINAL, PT 2

1

Match the enzymes with their function.
A. DNA polymerase I
B. DNA polymerase III
C. Primase
D. DNA ligase
E. DNA topoisomerase
F. Helicase
G. Single strand binding protein

In a newly synthesized prokaryotic DNA strand, the primers are removed by _____.

1

Match the enzymes with their function.
A. DNA polymerase I
B. DNA polymerase III
C. Primase
D. DNA ligase
E. DNA topoisomerase
F. Helicase
G. Single strand binding protein

_____ synthesizes DNA during replication.

1

Match the enzymes with their function.
A. DNA polymerase I
B. DNA polymerase III
C. Primase
D. DNA ligase
E. DNA topoisomerase
F. Helicase
G. Single strand binding protein

_____ require ATP to add negative supercoils to DNA.

1

Patients with the rare genetic disease xeroderma pigmentosum (XP) are very sensitive to light and are highly susceptible to skin cancers. XP is caused by defective DNA repair _____.

1

Match the following types of DNA damage to DNA repair system responsible for their repair.

1

What type of mutation most likely results from the alkylation of the oxygen on position 6 of guanine base producing O6-methylguanine?

1

Cigarette smoking has deleterious effects on reproduction. Which of the following statements is FALSE?

1

All of the following are true about nucleotide excision repair EXCEPT:

1

Which of the following mechanisms does NOT affect the mutation rate due to oxidation of guanine to 8-oxo-G?

1

Toxic cyanides are conjugated with:

1

Biotransformation by oxidation is catalyzed by:

1

How would you expect DNA methylation to alter gene expression?

1

What type of enzyme would most likely promote increased gene expression?

1

What characteristics of DNA allows methylation patterns to be maintained through replication and cell division?

1

What effect does histone acetylation have on transcription?

1

All are characteristics of RNA-directed DNA polymerase EXCEPT:

1

The point where separation of the DNA occurs is called the replication fork.

1

The primers required to initiate DNA replication remain in the DNA molecule after replication.

1

The PROOFREADING activity of E. coli DNA polymerases:

1

The enzyme activity inhibited by acyclovir is:

1

Acyclovir:

1

The flow of genetic information in Central Dogma of Biology (included the retroviruses) is as follows:

1

In order for an RNA virus such as HIV to direct synthesis of new viral RNA, it must first direct synthesis of DNA. In order to do this, the virus must violate the central dogma by using an enzyme called:

1

DNA polymerase can add new nucleotides to the free _____ of a nucleotide strand.

1

In a newly synthesized prokaryotic DNA strand, the primers are removed by _____.

1

DNA replicates through what process?

1

Zidovudine (AZT) is a more effective inhibitor of HIV reverse transcriptase than of mammalian cell DNA polymerases because:

1

Which of the following is NOT a function of DNA polymerase I?

1

During replication, DNA strands separate, causing supercoiling. These supercoils are removed by:

1

An apyrimidinic or apurinic (AP) site forms in which type of DNA repair?

1

In the first step of base excision repair

1

What modification neutralizes the charges on histones and loosens up the interactions between histones and DNA?

1

DNA methylation may be a significant mode of genetic regulation in eukaryotes. Methylation refers to

1

Which of the following enzyme(s) excision repair?

1

Which of the following is NOT TRUE of DNA methylation?

1

How does histone acetylation affect chromatin structure?

1

Nuclear receptors (estrogen receptor) belong to which class of transcription factor?

1

Co-repressor complex has the ability to decrease histone acetylation and represses transcription.

1

Which of the following statements are FALSE?

1

Match the DNA damage to DNA repair system responsible for their repair (using structure attached).

1

Old (parental) and new (daughter) strands of DNA in bacteria can be distinguished by

1

The reposition of helix-12 by estrogens (estradiol) creates an activation function (AF)-2 surface that allows the binding of coactivators LxxLL recognition site.

1

When the base cytosine undergoes deamination, it becomes

1

E. coli DNA pol I has all of the following characteristics EXCEPT:

1

Which of the following description about topoisomerases (gyrases) is FALSE?

1

AZT, is the first approved HIV drug. Which of the following statements about AZT are TRUE?

I. AZT is phosphorylated by cellular thymidine kinase to form AZT-monophosphate.
II. AZT-monophosphate is further phosphorylated by cellular kinases to form AZT-triphosphate.
III. AZT-triphosphate is a substrate for DNA polymerase, incorporated into growing polynucleotide chains in the place of adenosine residues.

1

A ten-year old girl is brought to the dermatologist by her parents. She has many freckles on her face, neck, arms, and hands, and the parents report that she is usually sensitive to sunlight. Two basal cell carcinomas are identified on her face. Which of the following processes is most likely to be defective in this patient?

1

All of the following are true about nucleotide excision repair EXCEPT:

1

Another type of DNA repair is base excision repair. Base excision repair

1

Cigarette smoking has deleterious effects on reproduction. Which of the following statements is FALSE?

1

Mutation in DNA may result from all of the following EXCEPT:

1

Tamoxifen is a selective estrogen receptor modulator, has been approved by the FDA for use in the treatment of breast cancer. Which of the following statements is FALSE?

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BCM-FINAL, PT 2

BCM-FINAL, PT 2

Match the enzymes with their function. A. DNA polymerase I B. DNA polymerase III C. Primase D. DNA ligase E. DNA topoisomerase F. Helicase G. Single strand binding protein In a newly synthesized prokaryotic DNA strand, the primers are removed by _____.

Match the enzymes with their function. A. DNA polymerase I B. DNA polymerase III C. Primase D. DNA ligase E. DNA topoisomerase F. Helicase G. Single strand binding protein _____ synthesizes DNA during replication.

Match the enzymes with their function. A. DNA polymerase I B. DNA polymerase III C. Primase D. DNA ligase E. DNA topoisomerase F. Helicase G. Single strand binding protein _____ require ATP to add negative supercoils to DNA.

Patients with the rare genetic disease xeroderma pigmentosum (XP) are very sensitive to light and are highly susceptible to skin cancers. XP is caused by defective DNA repair _____.

Match the following types of DNA damage to DNA repair system responsible for their repair.

What type of mutation most likely results from the alkylation of the oxygen on position 6 of guanine base producing O6-methylguanine?

Cigarette smoking has deleterious effects on reproduction. Which of the following statements is FALSE?

All of the following are true about nucleotide excision repair EXCEPT:

Which of the following mechanisms does NOT affect the mutation rate due to oxidation of guanine to 8-oxo-G?

Toxic cyanides are conjugated with:

Biotransformation by oxidation is catalyzed by:

How would you expect DNA methylation to alter gene expression?

What type of enzyme would most likely promote increased gene expression?

What characteristics of DNA allows methylation patterns to be maintained through replication and cell division?

What effect does histone acetylation have on transcription?

All are characteristics of RNA-directed DNA polymerase EXCEPT:

The point where separation of the DNA occurs is called the replication fork.

The primers required to initiate DNA replication remain in the DNA molecule after replication.

The PROOFREADING activity of E. coli DNA polymerases:

Match the enzymes with their function.
A. DNA polymerase I
B. DNA polymerase III
C. Primase
D. DNA ligase
E. DNA topoisomerase
F. Helicase
G. Single strand binding protein

In a newly synthesized prokaryotic DNA strand, the primers are removed by _____.

Match the enzymes with their function.
A. DNA polymerase I
B. DNA polymerase III
C. Primase
D. DNA ligase
E. DNA topoisomerase
F. Helicase
G. Single strand binding protein

_____ synthesizes DNA during replication.

Match the enzymes with their function.
A. DNA polymerase I
B. DNA polymerase III
C. Primase
D. DNA ligase
E. DNA topoisomerase
F. Helicase
G. Single strand binding protein

_____ require ATP to add negative supercoils to DNA.