Eva Bonning
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Medicine (CELS 191) Quiz am CELS 191 Lecture 16, erstellt von Eva Bonning am 24/05/2017.

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Eva Bonning
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CELS 191 Lecture 16

Frage 1 von 20

1

Select the statements that apply to prokaryotic DNA

Wähle eine oder mehr der folgenden:

  • Thy have a single, circular chromosome

  • There is a single point of origin for DNA replication

  • DNA replication is bidirectional

  • They have lots of linear DNA

  • There are multiple points of origin

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Frage 2 von 20

1

Select the statements that apply to eukaryotic DNA

Wähle eine oder mehr der folgenden:

  • They have lots of linear DNA

  • There are multiple points of origin for DNA replication

  • DNA replication is bidirectional

  • There is one, circular chromosome

  • There is one point of origin for DNA replication

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Frage 3 von 20

1

What is the role of the primase enzyme in DNA replication

Wähle eine der folgenden:

  • It adds RNA primers to the template strand

  • It unzips the DNA strand

  • It releases the tension at the DNA fork

  • It join synthesised fragments with phosphodiester bonds

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Frage 4 von 20

1

What is the role of the DNA Polymerase III enzyme in DNA replication

Wähle eine der folgenden:

  • It adds complimentary nucleotides from the reactive 3' hydroxyl group left by the RNA primer

  • It adds complimentary base pairs from the reactive 3' phosphate group left by the RNA primer

  • It binds at the 5' end and synthesises the new strand by adding base pairs

  • It binds at the 3' end and synthesises the new strand by adding phosphodiester bonds

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Frage 5 von 20

1

What is the role of the Helicase enzyme in DNA replication

Wähle eine der folgenden:

  • It unwinds the DNA from the AT rich point of origin

  • It unwinds the DNA from the point of origin

  • It unwinds the DNA from the CG rich point of origin

  • It puts the DNA back together after synthesis

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Frage 6 von 20

1

What is the role of the Topoisomerase enzyme in DNA replication

Wähle eine der folgenden:

  • It releases the tension at the replication fork by making cuts and then 'glues' them back together

  • It binds to the DNA to stop the leading and lagging strand fro getting back together

  • It unzips the gene

  • It checks the newly added bases against the template to make sure they are correct

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Frage 7 von 20

1

What is the role of single stranded binding proteins in DNA replication

Wähle eine oder mehr der folgenden:

  • They protect the single strands from degradation

  • They prevent the single strands from coming back together again

  • They make RNA primers

  • The release the tension at the replication fork by making cuts and then gluing them back together again

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Frage 8 von 20

1

What is the role of Ligase in DNA replication

Wähle eine der folgenden:

  • It joins synthesised fragments with phosphodiester bonds

  • It unzips the gene

  • It releases the tension at the replication fork

  • It makes RNA primers

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Frage 9 von 20

1

The overall direction of DNA synthesis on the lagging strand is in the 3'-5' direction

Wähle eins der folgenden:

  • WAHR
  • FALSCH

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Frage 10 von 20

1

How are errors in DNA sequence corrected during replication?

Wähle eine der folgenden:

  • With exonuclease activity of DNA Polymerase III

  • With endonuclease activity of DNA polymerase III

  • With exonuclease activity of DNA Polymerase II

  • With endonuclease activity of DNA polymerase II

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Frage 11 von 20

1

DNA replication, errors in DNA sequence are corrected with activity of DNA polymerase . It checks the newly added bases against the , removes it and replaces it so synthesis can continue as normal.

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    exonuclease
    endonuclease
    III
    II
    template
    lagging strand
    During
    After

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Frage 12 von 20

1

DNA replication, any errors in the sequence are corrected by activity of DNA polymerase . it removes the incorrect bases and some either side, corrects the mistake, then the newly synthesised sequence to the pre-existing sequence with .

Klicke und ziehe, um den Text zu vervollständigen.

    After
    During
    endonuclease
    exonuclease
    I
    III
    DNA polymerase
    Ligase
    joins
    detaches
    phosphodiester bonds
    hydrogen bonds

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Frage 13 von 20

1

Fülle die Lücken, um den Text zu vervollständigen.

Failure to mistakes in DNA sequence will lead to changes in sequence, in turn leading to a

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Frage 14 von 20

1

Fülle die Lücken, um den Text zu vervollständigen.

PCR (P C R) a gene so you can have copies of the gene. There are no strands (unlike prokaryotic replication) as both strands are synthesised .

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Frage 15 von 20

1

Select the key components of PCR

Wähle eine oder mehr der folgenden:

  • DNA template

  • Primers

  • Heat stable DNA polymerase

  • dNTPS

  • Buffer solution

  • Divalent Cations

  • Covalent Anions

  • RNA template

  • ddNTPS

  • DNA polymerase

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Frage 16 von 20

1

At what temperature does Denaturation of double stranded DNA occur during PCR?

Wähle eine der folgenden:

  • 95C

  • 45-70C

  • 72C

  • 85C

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Frage 17 von 20

1

At what temperature does Annealing of DNA templates occur during PCR?

Wähle eine der folgenden:

  • 95C

  • 45-70C

  • 72C

  • 105C

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Frage 18 von 20

1

At what temperature does extension/elongation occur during PCR?

Wähle eine der folgenden:

  • 72C

  • 95C

  • 50C

  • 40-75C

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Frage 19 von 20

1

What is the role of DNA polymerase I during DNA replication

Wähle eine oder mehr der folgenden:

  • It removes the RNA primers on Okazaki fragments

  • It fills in the gaps left by removed RNA primers with complimentary nucleotides

  • It adds the RNA primers on Okazaki fragments

  • It fills in the gaps left by removed DNA primers with RNA

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Frage 20 von 20

1

Fülle die Lücken, um den Text zu vervollständigen.

DNA replication begins with unwinding the DNA strands from an rich origin of replication, with (SSBP) protecting the strands from and stopping them from coming back together again. Topoisomerase releases at the replication fork by cutting and gluing the strands back together again. adds an primer with a chemically reactive ’ hydroxyl group, then DNA Polymerase adds complimentary from that hydroxyl group. On the leading strand, synthesis happens in the direction, however on the lagging strand this occurs in the overall direction in fragments. RNA primers are added intermittently along the strand, with DNA Polymerase III adding the complementary nucleotides in the 5’-3’ direction. DNA Polymerase then comes and removes the RNA primers on the leading and lagging strands, and fills in the gaps with nucleotides. forms the bonds between 3’ hydroxyl and 5’ phosphate groups, completing DNA synthesis.

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