1835758
Frage 1
Frage
What type of chromosome does G-banding "line-up"?
Antworten
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Metaphase
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Anaphase
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Telophase
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Prophase
Frage 2
Frage
What stain does G-banding use?
Antworten
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Giemsa
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Chrome red
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Methyl-blue
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Green
Frage 3
Frage
G-banding can only detect large abnormalities. True or false?
Antworten
- True
- False
Frage 4
Frage
What does FISH stand for?
Antworten
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Fluorescent in situ hybridization
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Fluorescent in site hydrolysis
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Familial in situ hybridization
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Familial in situ hydrolysis
Frage 5
Frage
You don't need to know what abnormality you are looking for when using FISH. True or false?
Antworten
- True
- False
Frage 6
Frage
Why do you need to know what abnormality you are looking for when using FISH?
Antworten
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Because it uses fluorescent probes to bind to specific sequences
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Because it used restriction enzymes marked with fluorescent probes to cut DNA at a specific sequence
Frage 7
Frage
How can you tell if there is an abnormality using the FISH technique?
Antworten
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Because some of the markers will not light up as they have not been able to anneal to bits of DNA sequence as they are missing
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Because all of the markers will light up because there are specific sequences of DNA that cause genetic disorders
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Because if there were no abnormalities the markers would not show up at all
Frage 8
Frage
FISH can show slightly smaller abnormalities than G-banding, but they still have to fairly large. True or false?
Antworten
- True
- False
Frage 9
Frage
What does QF-PCR stand for?
Antworten
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Quantitative fluorescence Polymerase Chain Reaction
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Qualitative fluorescence polymerase chain reaction
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Quantitative familial polymerase chain reaction
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Qualitative familial polymerase chain reaction
Frage 10
Frage
What is QF-PCR used to detect?
Antworten
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Trisomy syndromes
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Abnormalities in chromosome size
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Deletion syndromes
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Abnormalities in chromosome banding
Frage 11
Frage
What is the name for trisomy 13?
Antworten
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Pattau's syndrome
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Edward's syndrome
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Down's syndrome
Frage 12
Frage
What is the name for trisomy 18?
Antworten
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Pattau's syndrome
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Edward's syndrome
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Down's syndrome
Frage 13
Frage
What is the name for trisomy 21?
Antworten
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Pattau's syndrome
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Edward's syndrome
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Down's syndrome
Frage 14
Frage
Which biological technique is quickest?
Antworten
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FISH
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G-banding
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QF-PCR
Frage 15
Frage
You have to know what abnormality you are looking for when using QF-PCR. True or false?
Antworten
- True
- False
Frage 16
Frage
What sort of abnormalities does array-CGH detect?
Antworten
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Larger abnormalities
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Smaller abnormalities
Frage 17
Frage
What is DNA labelled with in array-CGH?
Antworten
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Fluorescent dye
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Coloured markers
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Radiation
Frage 18
Frage
What does the CGH in array-CGH stand for?
Antworten
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Comparative genomic hybridization
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Comparative genomic hydrolysis
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Comparative genetic hybridization
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Comparative genetic hydrolysis
Frage 19
Frage
How can you tell if there is an abnormality in the DNA when using array-CGH?
Antworten
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The relative fluorescence of the sample and control strand will be different
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The relative radiation of the sample and control strand will be different
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The length of the sample and control strand will be different when run through gel electrophoresis
Frage 20
Frage
Which two molecular biological techniques look at the actual base sequence when looking for genetic abnormalities?
Antworten
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G-banding
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FISH
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QF-PCR
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Array-CGH
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Sanger sequencing
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Next generation sequencing
Frage 21
Frage
Which of the following is cheaper and less time consuming?
Antworten
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Sanger sequencing
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Next generation sequencing
Frage 22
Frage
Why do we only sequence exons when using sanger sequencing?
Antworten
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Because the introns do not code for proteins so are not important to us
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Because it is time consuming and expensive
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Because introns rarely have mutations that cause genetic diseases
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Because we are unable to isolate the introns to sequence them
Frage 23
Frage
What machine is used in sanger sequencing to read the sequence of bases?
Antworten
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An electropheragram
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A Geiger counter
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A microscope
Frage 24
Frage
Not all changes to the base sequence cause disease. True or false?
Antworten
- True
- False
Frage 25
Frage
Why is it better to use next generation sequencing when you don't know what you are looking for?
Antworten
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Because it is easy to sequence a whole genome
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Because sanger sequencing would take too long
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Because next generation sequencing is more accurate
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Because next generation sequencing is less expensive
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