456291
Beschreibung
Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor mehr als 10 Jahre
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Frage 1
Frage
Gene synthesis can be fully automated cheaply
Antworten
- True
- False
Frage 2
Frage
Why is genome synthesis difficult?
Antworten
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Chemical synthesis is limited to 100nt at a time
-
Manipulation of large fragments is difficult
-
Vectors are limited
Frage 3
Frage
Synthesis of a genome must be done in small, overlapping parts
Antworten
- True
- False
Frage 4
Frage
How many kbpairs can PCR be used for?
Antworten
-
1-5
-
5-10
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10-15
Frage 5
Frage
What is essential when creating small fragments to be joined together later in clones?
Antworten
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The fragments must overlap
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The fragments MUST be 7kb long
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The fragments cannot contain introns
Frage 6
Frage
When transplacting the synthetic genome between clones, what must not be present?
Antworten
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Proteins
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DNA
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RNA
Frage 7
Frage
How is the receptor organism for the synthetic genome made able to receive it?
Antworten
-
The cell wall is treated with chemicals to make it permeable
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The DNA is directly microinjected into the cell
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The cell absorbs the DNA with other nutrients
Frage 8
Frage
Once the synthetic genome has been added to the cell, it divides as it contains two genomes
Antworten
- True
- False
Frage 9
Frage
Why synthetic organisms?
Antworten
-
To see if we can
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To improve/redesign regulatory networks
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To produce protective genes
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Biofuels/biopharmecuticals
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