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Quiz von gina_evans0312, aktualisiert more than 1 year ago
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Erstellt von gina_evans0312
vor mehr als 10 Jahre
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Frage 1
Frage
A radioactive probe can take what forms?
Antworten
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ssDNA
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RNA
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Denatured dsDNA
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Oligonucleotides
Frage 2
Frage
Probes can be
Antworten
-
Radioactive
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Fluorescent
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Use Ig's
Frage 3
Frage
Name the two ways probes can be designed
Antworten
-
Guesswork
-
Codon Bias
Frage 4
Frage
What is codon bias?
Antworten
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Where cells prefer one codon of an aa to another
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Where cells will reject certain codons due to what they contain
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Where some cells can only make tRNA's for certain codons
Frage 5
Frage
80% of Phe codons are TTC, 20% are TTT
Antworten
- True
- False
Frage 6
Frage
If the protein is an enzyme it can be found by adding the substrate
Antworten
- True
- False
Frage 7
Frage
State the possible forms of Ig's for protein separation?
Antworten
-
Immobalised on a column
-
Removable from solution
Frage 8
Frage
Probes needn't be 100% accurate to work
Antworten
- True
- False
Frage 9
Frage
For a Western Blot, how is the DNA stuck to the membrane?
Antworten
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Denatured DNA sticks to membrane
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Bacteria are lysed to release DNA
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DNA washes off
Frage 10
Frage
Once the DNA has been bound, what happens next?
Antworten
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A probe is added
-
The membrane is run through a gel
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The membrane is stained
Frage 11
Frage
In an expression vector, where is the new gene added?
Antworten
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The Multiple Cloning Site
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The Promotor Region
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Next to the origin
Frage 12
Antworten
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Origin
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Antibiotic Resistance
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Promoter
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Terminator
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Multiple Cloning Site
Frage 13
Frage
More compliated vectors have proteins tagged for purification
Antworten
- True
- False
Frage 14
Frage
What is involved in His Chain Purification?
Antworten
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The addition of a His chain to the N terminus
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The removal of a His chain from the protein
-
The use of a His chain to cut unwatned proteins
Frage 15
Frage
His Purification uses Histadine's affinity for what metal?
Antworten
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Ni2+
-
Fe2+
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Na+
Frage 16
Frage
His-tagged proteins will bind to Nickel in a column. Others that don't bind will be washed off.
Antworten
- True
- False
Frage 17
Frage
The PET system involves using a promoter for the protein that you can control the expression of
Antworten
- True
- False
Frage 18
Frage
What promoter is used in the PET system to control protein expression?
Antworten
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T7
-
T8
-
T9
Frage 19
Frage
In the PET system, what promoter is used to control the T_ expression?
Antworten
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Lac promoter
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Slo Promoter
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Pap promoter
Frage 20
Frage
What is required for a good shuttle vector?
Antworten
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Replicable in both hosts
-
Selectable in both hosts
-
Expressable in both hosts
Frage 21
Frage
Shuttle vectors are usually plasmids with additional sequences
Antworten
- True
- False
Frage 22
Frage
Shuttle vectors for yeast/fungi need
Antworten
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Special origin
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Antibiotic/metabolic marker
Frage 23
Frage
A baclovirus would be used as a vector into what?
Antworten
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Insects
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Plants
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Mammals
Frage 24
Frage
Secretion of certain proteins can be caused by what?
Antworten
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Disulphide bonds
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Acetylation
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Methylation
Frage 25
Antworten
-
One for the yeast to use
-
One for the bacteria to use
Frage 26
Antworten
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URA3- allows growth in the absence of Uracil
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TRA3- allows growth in the absence of thymidine
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GRA3- allows growth in the absence of guanine
Frage 27
Frage
Like bacteria, yeast cannot take up linear DNA
Antworten
- True
- False
Frage 28
Frage
What does a YAC contain that a plasmid does not?
Antworten
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Non-useful genes
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Yeast centromere
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Yeast telomeres
Frage 29
Frage
Yeast can allow DNA to be directly inserted into its genome
Antworten
- True
- False
Frage 30
Frage
How can yeast accept DNA into its genome?
Antworten
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Homologous recombination
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Non-homologous end joining
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Direct injection
Frage 31
Frage
In order to directly insert a gene, you must flank the gene with identical stretches of the part of the genome you want to insert it in
Antworten
- True
- False
Frage 32
Frage
Plants would use an aglobacterium T1 plasmid
Antworten
- True
- False
Frage 33
Frage
How do you replicate transformed plant cells?
Antworten
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Grown them as plantlets
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Clone them
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Asexually reproduce them
Frage 34
Frage
Mammal shuttle vectors are the same as yeast vectors, but the yeast origin is replaced with what?
Antworten
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An SV40 promoter
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A UG35 promoter
-
A FT12 promoter
Frage 35
Frage
Retroviruses can also be used to insert transgenes into mammilian genomes by packing the transgene in a retroviral capsule
Antworten
- True
- False
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