What is a muscle physiologist interested in?
What happens during a molecular adaptation?
What affects whether a sensor protein is active or inactive?
What does this lead to?
Where are messages taken to make proteins?
Where does this occur?
How many types of cell are there?
What determines the function of a cell?
What is the first stage of adaptation?
What is cell signalling/transduction?
What creates extracellular molecules?
What are they called?
Give some examples
Ligands include a chain reaction of signalling proteins, what is that chain?
Describe the simplistic cell signalling diagram.
What do intracellular signalling proteins act as?
What does a molecular switch do?
What is it undertaken by?
How can we assess signalling muscle?
How do we stop phosphorylation occuring?
What is protein quantification assay?
What does the assay contain?
What two reactions does the protein quantification assay rely on?
If there is more protein when protein quantification assay is performed, what will happen?
How do you prepare a sample for protein quantification assay? (step 1&2)
How do you prepare a sample for protein quantification assay? (step 3,4 & 5)
Describe SDS PAGE.
What do you do with the proteins on the gel after SDS PAGE?
When using SDS PAGE what is used as the control and why?
What is a primary antibody?
What do you do with a primary antibody?
What is a secondary antibody?
How do you detect luminol?
What is a transcription factor?
Where does it occur?
What does it do?
What does RNA polymerase do?
How do we isolate RNA?
After centrifuging RNA isolation what are the phases presented?
What is RNA made up of?
What is special about them and why?
Why are nucleic acids soluble in the upper aqueous phase instead of the lower organic phase?
What is alcohol precipitation?
What does PCR detect?
What is RT-PCR reverse transcription?
Why is this necessary?
What are the steps of a polymerase chain reaction after a double strand has been created? (1-3)
What are the steps of a polymerase chain reaction after a double strand has been created? (4&5)
Overview: structural, compositional & cellular adaptation
Immuno-histochemistry (IHC) fibre type
Bioengineered skeletal muscle
Summary
What are the three pillars of molecular adaptation?
