Microbiology Lab Practical

Question 1 of 127

1

What method of staining was used to create this slide?

Select one of the following:

Acid-fast Stain
Endospore Stain
Gram Stain
Negative Stain

Explanation

Question 2 of 127

1

What kind of staining process was used to make this slide?

Select one of the following:

Negative Stain
Endospore Stain
Gram Stain
Acid-fast Stain

Explanation

Question 3 of 127

1

What kind of microbes are present on this slide?

Select one of the following:

Gram (-)
Endospores
Nocardia
Mycobacterium

Explanation

Question 4 of 127

1

Where should your backpacks and purses be stored during the lab period?

Select one of the following:

On the shelf under the lab bench.
On the floor next to your seat.
On the lab bench.

Explanation

Question 5 of 127

1

What items should you wear to protect yourself in the lab.

Select one or more of the following:

Lab coat
Safety Glasses
Gloves
Closed toe shoes
Hair net
Shoe covers

Explanation

Question 6 of 127

1

When do you disinfect your lab table and where do you discard the used paper towels?

Select one or more of the following:

Disinfect on the way into the lab.
Disinfect on the way out of the lab.
Paper towels go in the regular trash.
Paper towels go in the autoclave trash.
Disinfect between each step of the lab process.

Explanation

Question 7 of 127

1

You are dying of thirst (hunger, for smoke, chapped lips, bad breath) during the lab period. What do you do to alleviate your problem?

Select one of the following:

Leave
Disinfect your lab bench, take care of your need and then disinfect your lab bench, again.
Go to the corner of the lab room and take care of your need.

Explanation

Question 8 of 127

1

You notice your lab partner has caught the lab manual on fire. What should you do?

Select one or more of the following:

Alert the professor
Get the fire extinguisher near the front door
Get the fire extinguisher from the back of the room
Get deionized water from the back of the room and pour it on the fire

Explanation

Question 9 of 127

1

Your lab partner has managed to spill microorganisms all over the lab table. What should be done to clean up the spill?

Select one or more of the following:

Alert the professor
Spray disinfectant on the spill
Spray disinfectant on a paper towel and place on the spill
Keep spraying disinfectant on the paper towel
Wait 10 minutes
Wait 15 minutes
Dispose in autoclave trash
Dispose in regular trash

Explanation

Question 10 of 127

1

A student broke an empty glass test tube. What should be done to clean up the glass and where should the pieces of glass be disposed of?

Select one or more of the following:

Clean with dust pan and broom next to the fridge.
Clean with dust pan and broom near the front door.
Dispose of in the broken glass disposal box.
Dispose of in the autoclave trash.
Dispose of in the regular trash.

Explanation

Question 11 of 127

1

The instructor tripped on a backpack on the floor knocking her safety goggles off and splashing HCl in her eyes. What should she do?

Select one or more of the following:

Use the eyewash located near the front door.
Use the eyewash located at the back of the room.
Use the eyewash next to the fridge.
Use the eyewash near back of the room.

Explanation

Question 12 of 127

1

After surviving the lab period you remove your gloves by turning them inside out and then where do you toss them?

Select one or more of the following:

In the autoclave trash
In the regular trash
In the used glove disposal bin

Explanation

Question 13 of 127

1

Choose the two major types of microscopes that have been developed and the difference between them

Select one or more of the following:

Compound and Electron.
Compound uses light and Electron uses electrons.
Compound and Bright Field.
Compound uses electrons and Bright Field uses light.
Compound uses light and Bright Field uses electrons.

Explanation

Question 14 of 127

1

Which microscopes are compound microscopes?

Select one or more of the following:

Phase/Contrast Microscope
Bright Field Microscope
Dark Field Microscope
Fluorescent Microscope
Scanning Microscope
Objective Microscope

Explanation

Question 15 of 127

1

The ability of the microscope to enlarge an object.

Select one of the following:

Magnification
Resolving power.
Refractive index.
Resolution.

Explanation

Question 16 of 127

1

How clear your specimen will appear when viewed through the lenses.

Select one of the following:

Resolution
Refractive index
Resolving power
Magnification

Explanation

Question 17 of 127

1

How well you lens is able to bend light.

Select one of the following:

Refractive index.
Resolution
Resolving power
Magnification

Explanation

Question 18 of 127

1

What components influence the resolving power of a microscope?

Select one or more of the following:

Numerical aperture
Wavelength of light
Condenser
Diopter ring

Explanation

Question 19 of 127

1

Concentrates the light beam and directs light through the specimen.

Select one of the following:

Condenser
Iris diaphragm
Diopter ring
Refractive index

Explanation

Question 20 of 127

1

Regulates the intensity of the light entering the lens.

Select one of the following:

Iris diaphragm
Condenser
Diopter ring
Rheostat

Explanation

Question 21 of 127

1

Focuses the individual eye piece to your eye.

Select one of the following:

Diopter ring
Rheostat
Diaphragm ring
Iris diaphragm

Explanation

Question 22 of 127

1

Steps to achieve adequate lighting when the oil immersion objective is used to view a specimen.

Select one or more of the following:

Condenser raised to the highest position
Iris and base diaphragm are open
Rheostat is turned up
Condenser is lowered to the lowest position

Explanation

Question 23 of 127

1

Components that influence the resolving power of a microscope.

Select one or more of the following:

Numerical aperture
Wavelength of light
Rheostat level
Refractive index

Explanation

Question 24 of 127

1

The isolation of a single species of microbe.

Select one of the following:

Pure culture
Colony
Media
Inoculum
Turbdity

Explanation

Question 25 of 127

1

Cells from a single asexual parent that are identical.

Select one of the following:

Colony
Pure culture
Media
Inoculum
Turbidity

Explanation

Question 26 of 127

1

Nutrient material suitable for the cultivation of microorganisms.

Select one of the following:

Media
Pure culture
Colony
Turbdity
Inoculum

Explanation

Question 27 of 127

1

Transfer of a pure culture from a slant culture to a new sterile broth. The sampling of a bacterial culture.

Select one of the following:

Inoculum
Pure culture
Colony
Media
Turbidity

Explanation

Question 28 of 127

1

Cloudiness in broth that indicates growth.

Select one of the following:

Turbidity
Pure culture
Media
Colony
Inoculum

Explanation

Question 29 of 127

1

Steps involved in labeling a test tube.

Select one of the following:

Label a piece of labeling tape with your name and the name of the bacteria and place on test tube.
Use a wax pencil and label the test tube with your name and the name of the bacteria.
Use a marker and label the test tube with your name and the name of the bacteria.

Explanation

Question 30 of 127

1

What are the steps for labeling a Petri plate?

Select one of the following:

Label the bottom of the media side using a wax pencil or marker with your name and the organism you are transferring.
Label the lid using a wax pencil or marker with your name and the name of the organism you are transferring.
Label a piece of labeling tape with your name and the name of the organism you are transferring and place it on the lid of the Petri plate.

Explanation

Question 31 of 127

1

What are the steps for incubating a Petri plate?

Select one of the following:

Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side up.
Sterilize inoculating loop in Bacticinerator. Lightly drag the tip of the loop from side to side or back and forth in order to spread the inoculum. Replace lid. Incubate lid side down.

Explanation

Question 32 of 127

1

What is meant by the statement that microorganisms are ubiquitous?

Select one of the following:

Microorganisms are everywhere
Microorganisms are hard to identify
Microorganisms are difficult to see without a microscope
Microorganisms come in many forms

Explanation

Question 33 of 127

1

Handling of bacteria in a manner so as to prevent contaminating laboratory media and to prevent infecting ourselves and our classmates.

Select one of the following:

Aseptic technique
Sterile transfer
Inoculation technique
Ubiquitous

Explanation

Question 34 of 127

1

Why are "basic" dyes used in the Gram stain?

Select one of the following:

Bacteria have an overall negative charge so stain with positive (basic) dyes. Opposites attract.
Bacteria have an overall positive charge so stain with positive (basic) dyes. Likes attract likes.

Explanation

Question 35 of 127

1

State two differences between a simple stain and a differential stain.

Select one of the following:

Simple stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Differential stain uses two dyes; different bacteria are stained contrasting colors; shows size, shape, arrangement and something else.
Differential stain uses one dye; all bacteria are the same color; shows size, shape and arrangement. Simple stain uses two dyes; different bacteria are stained contrasting colors for simple identification; shows size, shape, arrangement and something else.

Explanation

Question 36 of 127

1

A) Has a high lipid content and thin layer of tecoic acid underneath and stains pink.
B) Has a low lipid content and thick layer containing tecoic acids and stains purple.

Select one of the following:

A) Gram negative bacteria
B) Gram positive bacteria
A) Gram positive bacteria
B) Gram negative bacteria
A) Gram stain
B) Acid-fast stain
A) Acid-fast stain
B) Gram stain

Explanation

Question 37 of 127

1

What us the importance of using only a pinpoint of inoculum for the smear preparation?

Select one or more of the following:

Insure proper staining
Insure proper decolorizing
The slide is small
Allows you to prepare only one or two bacteria.

Explanation

Question 38 of 127

1

What is the importance of allowing the smear to air dry?

Select one or more of the following:

Insure the cells stay intact and stain properly.
Insure the cells are dead and stain properly
Insure the cells wont move around during staining

Explanation

Question 39 of 127

1

What is the importance of decolorizing smears one at a time?

Select one of the following:

It is the most critical step and there is no hard and fast rule on how long to decolorize.
It is the most critical step and don't want to drop the slides.
It is the most critical step and need to time it exactly.

Explanation

Question 40 of 127

1

List the gram stain reagents in order and state their function.

Select one of the following:

1) Crystal Violet - Primary stain
2) Iodine - Mordant, combines with the crystal violet in the cell.
3) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
4) Safranin - Counterstain or Secondary stain
1) Crystal Violet - Primary stain
2) Safranin - Counterstain or Secondary stain
3) Iodine - Mordant, combines with the crystal violet in the cell.
4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
1) Crystal Violet - Primary stain
2) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer
3) Iodine - Mordant, combines with the crystal violet in the cell.
4) Safranin - Counterstain or Secondary stain
1) Crystal Violet - Primary Stain
2) Iodine - Mordant, combines with the crystal violet in the cell
3) Safranin - Counterstain or Secondary stain
4) Acetone or ethanol or combination of 75% ethanol/25% acetone - Decolorizer

Explanation

Question 41 of 127

1

Why do gram negative cells lose the primary dye during decolorization?

Select one of the following:

The high lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.
The low lipid content in gram negative cell walls causes them to dissolve in the ethanol allowing the crystal violet dye to leak out.

Explanation

Question 42 of 127

1

Why is the Acid-fast stain considered a "differential stain"?

Select one or more of the following:

It distinguishes between two groups of organisms based on the lipid content in their cell walls.
It uses two positively charged dyes.
It uses one positively charged dye and one negatively charged dye.
It tells size, shape, arrangement and something else.
It tells size, shape and arrangement.

Explanation

Question 43 of 127

1

What is the difference between the decolorizer used in the Gram stain and the decolorizer used in the Acid-fast stain?

Select one of the following:

Acid fast uses a solution of 3% HCl and 95% ethanol. Gram stain uses just ethanol.
Acid fast uses a solution of 95% HCl and 3% ethanol. Gram stain uses just ethanol.

Explanation

Question 44 of 127

1

Lipids in the cell's waxy wall prevent the acid-alcohol from decolorizing the cell.

Select one of the following:

Acid-fast
Gram
Negative
Endospore

Explanation

Question 45 of 127

1

What is the difference between the Ziehl-Neelsen stain and the Kinyoun stain?

Select one of the following:

Ziehl-Neelsen uses steam to penetrate the mycolic acid in cell walls.
Kinyoun uses concentrated dye, hard heat fixing and longer exposure time.
Kinyoun uses steam to penetrate the mycolic acid in cell walls.
Ziehl-Neelsen uses concentrated dye, hard heat fixing and longer exposure time.
Ziehl-Neelsen uses hard heat fixing to penetrate the mycolic acid in cell walls.
Kinyoun uses concentrated dye, steam and longer exposure time.
Kinyoun uses hard heat fixing to penetrate the mycolic acid in cell walls.
Ziehl-Neelsen uses concentrated dye, steam and longer exposure time.

Explanation

Question 46 of 127

1

Which are acid-fast organisms and what disease do they cause?

Select one or more of the following:

Mycobacterium tuberculosis
Disease: Tuberculosis
Mycobacterium leprae
Disease: Leprosy or Hansen's disease
Nocardia asteroides
Disease: Nocardiosis
Cryptosporidium
Disease: Cryptosporidiosis
Mycosporidium
Disease: Mycardiosis
Nocardia tuberculosis
Disease: Tuberculosis

Explanation

Question 47 of 127

1

What are the reactants in the acid-fast stain, in order?

Select one of the following:

1) Carbolfuchsin
2) Acid-alcohol
3) Loeffler's methylene blue
1) Loeffler's methylene blue
2) Acid-alcohol
3) Carbolfichsin
1) Carbolfuschsin
2) Iodine
3) Acid-alcohol
4) Loeffler's methylene blue
1) Loeffler's methylene blue
2) Iodine
3) Acid-alcohol
4) Carbolfuchsin

Explanation

Question 48 of 127

1

Two methods used to force the dye into an endospore.

Select one or more of the following:

Steam for 3-5 minutes
Hard heat fixing then letting the dye remain on the slide for an extended period of time.
Hard heat fixing
Steam then letting the dye remain on teh slide for an extended period of time.

Explanation

Question 49 of 127

1

What dyes are used in the Schaffer-Fulton spore stain?

Select one or more of the following:

Malachite green
Gram's safranin
Iodine
Loeffler's methylene blue

Explanation

Question 50 of 127

1

In the "Christmas tree" stain, the endospores are colored _ and the vegetative cells are colored ____.

Select one or more of the following:

Green
Red
Red
Green

Explanation

Question 51 of 127

1

Metabolically active, replicating cell.

Select one or more of the following:

Vegetative cell
Endospore
Germinated cell

Explanation

Question 52 of 127

1

The formation of endospores within a vegetative cell

Select one of the following:

Sporogenesis
Decolorization
Germination

Explanation

Question 53 of 127

1

When an endospore reverts to a vegetative state.

Select one of the following:

Germination
Sporogenesis
Growth

Explanation

Question 54 of 127

1

Why are endospores considered "the most resistant life form known"?

Select one or more of the following:

Can remain dormant indefinitely
Resistant to antibiotics and most disinfectants
Resistant to radiation
Resistant to boiling
Resistant to drying
Impermeability of spore coat

Explanation

Question 55 of 127

1

What are the medically important endospore-producing bacteria and their associated disease?

Select one or more of the following:

Bacillus anthracis
Disease: anthrax
Clostridium botulinum
Disease: Botulism
Clostridium difficile
Disease: Toxic enterocolotis
Clostridium perfringens
Disease: Gas gangrene, food poisoning
Clostridium tetani
Disease: Tetnus, lockjaw
Clostridium anthracis
Disease: Anthrax

Explanation

Question 56 of 127

1

Which medically important genera of bacteria produce endospores?

Select one or more of the following:

Bacillus
Clostridium
Streptococcus
Staphylococcus

Explanation

Question 57 of 127

1

A positive stain _ and a negative stain __.

Select one of the following:

Stains what you want to see
Stains everything but the structure you want to see
Stains everything but the structure you want to see
Stains what you want to see
Stains gram (+) bacteria
Stains gram (-) bacteria
Stains gram (-) bacteria
Stains gram (+) bacteria

Explanation

Question 58 of 127

1

Why are Nigrosin and India ink commonly used negative stains?

Select one of the following:

They are negatively charged dyes and are repelled by the negative contents of the bacterial cell.
They are positively charged dyes and are repelled by the positive contents of the bacterial cell.
They are positively charged dyes and are attracted by the negative contents of the bacterial cell.
They are negatively charged dyes and are attracted by the positive contents of the bacterial cell.

Explanation

Question 59 of 127

1

What are the advantages of using a negative stain versus a positive stain?

Select one or more of the following:

Able to see a capsule
Do not heat fix so can see them as they are
Can be used for things that don't stain.
Can see if it produces endospores
Can see if it is gram (-) or gram (+)

Explanation

Question 60 of 127

1

Sticky substance that is tightly bound, highly organized and generally round or oval.

Select one of the following:

Capsule
Slime layer
Endospore

Explanation

Question 61 of 127

1

Sticky substance is loosely bound and somewhat irregularly shaped.

Select one of the following:

Capsule
Slime layer
Endospore

Explanation

Question 62 of 127

1

How does a capsule contribute to the virulence of a bacteria?

Select one or more of the following:

Helps the bacteria avoid phagocytosis by white blood cells
Helps the bacteria attach to skin and mucous membranes
Is a source of nutrition for the bacteria when resources are scarce
Contains the pathogenic part of the bacteria

Explanation

Question 63 of 127

1

What are the difference(s) between the smear preparation for a Gram stain and a capsule stain?

Select one or more of the following:

A smear for a capsule stain is not heat fixed.
A spear from a capsule stain is not rinsed with deionized water it is rinsed with copper sulfate
A smear for a capsule stain uses steam
A smear for a Gram stain uses crystal violet and a capsule stain does not

Explanation

Question 64 of 127

1

Using Anthony's stain, the background will be colored and the capsule will be colored _____.

Select one of the following:

Dark blue
Light blue to white
Light blue to white
Dark blue

Explanation

Question 65 of 127

1

What are the damaging by-products of oxygen during cellular respiration?

Select one or more of the following:

Superoxides
Peroxides
Carbon dioxide
Carbon monoxide

Explanation

Question 66 of 127

1

What enzymes neutralize the damaging effects of the by-products of oxygen during cellular respiration?

Select one or more of the following:

Catalase
Peroxidase
Superoxide dismutase
Carbonase

Explanation

Question 67 of 127

1

Uses oxygen as the final member of the ETC and has enzymes to break down the toxic by-products of oxygen.

Select one of the following:

Aerobe
Anerobe

Explanation

Question 68 of 127

1

Does not use oxygen as the final member of the ETC and lacks the enzymes to breakdown the toxic by-products of oxygen.

Select one of the following:

Aerobe
Anaerobe

Explanation

Question 69 of 127

1

How does a candle jar achieve an atmosphere to grow microaerophilic and capnophilic organisms?

Select one of the following:

The flame burns using up the oxygen and producing CO2
The flame burns up the CO2 and produces oxygen.

Explanation

Question 70 of 127

1

How does a Gas Pak jar achieve an anaerobic atmosphere?

Select one of the following:

The pak contains chemicals that produce H+ and CO2. The H+ combines with the O2 in the jar to produce H2O.
The pak contains chemicals that produce CO2 and destroy O2.

Explanation

Question 71 of 127

1

Which type of organism grows equally well in all types of oxygen concentrations?

Select one of the following:

Facultative anaerobes
Microaerophiles
Capnophiles
Aerotolerant anaerobes

Explanation

Question 72 of 127

1

How does thioglycolate broth grow anaerobic bacteria as well as aerobic bacteria?

Select one or more of the following:

The agar prevents diffusion of O2
It contains a reducing agent, sodium thioglycollate, that combines with O2 to produce water
It contains a reducing agent, sodium thioglycollate, that combines with the agar to produce CO2 near the bottom
The agar prevents diffusion of CO2

Explanation

Question 73 of 127

1

Organisms that require around 20% atmospheric oxygen.
(Check the name of the organism and the media on which it will grow.)

Select one or more of the following:

Strict aerobe
Facultative anaerobe
Only at the top of the thioglycollate broth.
Candle jar
Gas pak
Capnophile

Explanation

Question 74 of 127

1

Organisms that require between 5-15% atmospheric oxygen for growth
(Check the name of the organism and the media on which it will grow.)

Select one or more of the following:

Microaerophile
Facultative anaerobe
Grows in candle jar
Grows at the point where the oxygen level is diminishing in the thio broth.
Grows in the GasPak

Explanation

Question 75 of 127

1

Organisms that require between 5-15% atmospheric oxygen and increased (10%) carbon dioxide.
(Check the name of the organism and the media on which it will grow.)

Select one or more of the following:

Capnophile
Microaerophile
Grows in candle jar
Grows in oxygen
Grows in a GasPak

Explanation

Question 76 of 127

1

Organisms that will use a fermentative metabolic pathway in anaerobic conditions yet can convert to aerobic cellular respiration when atmospheric oxygen is present. They grow equally well in aerobic anaerobic environments.

Select one or more of the following:

Facultative anaerobe
Microaerophile
Grows in oxygen
Grows in candle jar
Grows in GasPak
Grows throughout the entire thio broth slant

Explanation

Question 77 of 127

1

Organism that can exist in the presence of oxygen, but does not use oxygen for metabolism.

Select one or more of the following:

Aerotolerant anaerobe
Microaerophile
All over the thio broth slant but slightly less at the top
Grows in the GasPak
Capnophile

Explanation

Question 78 of 127

1

Organisms that die in the presence of atmospheric oxygen because they lack the enzymes needed to breakdown the toxic by-products.

Select one or more of the following:

Strict anaerobe
Grows in GasPak
Grows in candle jar
Grows toward the bottom of the thio broth
Grows toward the top of the thio broth

Explanation

Question 79 of 127

1

Which gene allows jellyfish to glow in the dark?

Select one of the following:

Green fluorescent protein
Fluorescent blue plasmid
GFP
FBP

Explanation

Question 80 of 127

1

Bacteria cells able to take up the plasmid/DNA.

Select one of the following:

Competent cells
Competent genes
Competent proteins

Explanation

Question 81 of 127

1

What laboratory procedures are done to encourage cells to become competent?

Select one or more of the following:

Mixing cells with calcium chloride during growth phase
Exposing cells to extreme temperature changes during the proceduree
Mixing cells with with heated calcium hydroxide
Freezing cells

Explanation

Question 82 of 127

1

What is the relationship between a gene and a protein?

Select one of the following:

A gene is a piece of DNA that provides the instructions for making a protein
A protein is a piece of DNA that provides the instructions for making a gene
DNA is a piece of a protein that provides the instructions for making a gene

Explanation

Question 83 of 127

1

Chromosomal DNA _ and plasmids _______.

Select one of the following:

Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.
Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.
Small pieces of circular DNA that have genes for one or more traits that may be beneficial but are not essential for bacterial survival.
Carries genes needed for the hereditary characteristics essential for bacterial growth and reproduction.
Carries genes needed for the hereditary characteristics that may be beneficial but are not essential for bacterial growth and reproduction.
Small pieces of circular DNA that have genes for one or more traits that are essential for bacterial survival.

Explanation

Question 84 of 127

1

Which genes are contained in the pGLO plasmid?

Select one or more of the following:

GFP
BLA
Ara-C
Ara-B

Explanation

Question 85 of 127

1

How is expression of the Green Fluorescent Protein gene regulated?

Select one of the following:

The Ara-C gene controls expression of GFP via the sugar arabinose
The Ara-B gene controls expression of GFP via the sugar arabinose
The Ara-C gene controls expression of GFP via the sugar glucose

Explanation

Question 86 of 127

1

Chemicals produced by microorganisms that in small quantities can inhibit the growth of other microorganisms

Select one of the following:

Antibiotic
MIC
E-test

Explanation

Question 87 of 127

1

Determines the lowest concentration of an antibiotic that is able to inhibit the growth of a test organism.

Select one of the following:

Antibiotic
MIC
E-test

Explanation

Question 88 of 127

1

A plastic coated strip that contains a gradient

Select one of the following:

Antibiotic
MIC
E-test

Explanation

Question 89 of 127

1

What are the disadvantages of using a broad spectrum antibiotic?

Select one or more of the following:

Contributes to escalating drug resistance
Wipes out a person's normal flora resulting in superinfections
Only works on weak bacteria

Explanation

Question 90 of 127

1

What does the antibiotic test result "sensitive" indicate about the test organism?

Select one or more of the following:

An organism is susceptible to drug concentrations obtained in the body using normal therapeutic doses.
The antibiotic is likely to be effective against the pathogen
The antibiotic is not likely to work.

Explanation

Question 91 of 127

1

What factors must be controlled in order to standardize the Kirby-Bauer test?

Select one or more of the following:

Stability of the antibiotic
Rate of diffusion of the antibiotic
pH of the culture medium
Depth of the culture medium
Inoculum density
Incubation time
Incubation temperature
Concentration of antibiotic

Explanation

Question 92 of 127

1

A?

Select one of the following:

Arm
Rheostat
Power switch
Coarse focus knob

Explanation

Question 93 of 127

1

B?

Select one of the following:

Rheostat
Power switch
Coarse focus knob
Arm

Explanation

Question 94 of 127

1

C?

Select one of the following:

Rheostat
Power switch
Coarse focus knob
Fine focus knob

Explanation

Question 95 of 127

1

D?

Select one of the following:

Coarse focus knob
Fine focus knob
RHeostat
Power switch

Explanation

Question 96 of 127

1

E?

Select one of the following:

Fine focus knob
Coarse focus knob
Rheostat
Power switch

Explanation

Question 97 of 127

1

A?

Select one of the following:

Ocular
Diopter
Diaphragm ring
Fine focus knob

Explanation

Question 98 of 127

1

B?

Select one of the following:

Mechanical stage
Base
Slide stage
Ocular stage

Explanation

Question 99 of 127

1

C?

Select one of the following:

Fine focus knob
Coarse focus knob

Explanation

Question 100 of 127

1

D?

Select one of the following:

Base
Mechanical stage
Mechanical base

Explanation

Question 101 of 127

1

A
B
C
D
E

Select one of the following:

A - Objective lens
B - Iris diaphragm lever
C - Condenser centering screw
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Iris diaphragm lever
C - Condenser centering screw
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Condenser centering screw
C - Iris diaphragm lever
D - Base diaphragm
E - Mechanical stage knob
A - Ocular lens
B - Iris diaphragm lever
C - Mechanical stage knob
D - Base diaphragm
E - Condenser centering screw

Explanation

Question 102 of 127

1

A
B
C

Select one of the following:

A - Iris diaphragm lever
B - Condenser centering screw
C - Base diaphragm
A - Condenser centering screw
B - Iris diaphragm lever
C - Base diaphragm
A - Iris diopter screw
B - Iris diaphragm lever
C - Base diaphragm
A - Iris diopter lever
B - Condenser centering screw
C - Base diaphragm

Explanation

Question 103 of 127

1

A
B

Select one of the following:

A - Revolving nose piece
B - Mechanical stage clip
A - Objective ring
B - Mechanical stage clip
A - Diopter ring
B - Mechanical stage clip

Explanation

Question 104 of 127

1

A
B
C

Select one of the following:

A - Condenser knob
B - Condenser
C - Base diaphragm ring
A - Condenser knob
B - Condenser ring
C - Base
A - Condenser ring
B - Condenser
C - Base

Explanation

Question 105 of 127

1

In the thioglycollate test tube A, what kind of organism does the growth represent?

Select one of the following:

Microaerophile
Obligate/Strict aerobe
Facultative anaerobe
Obigate/strict anaerbe

Explanation

Question 106 of 127

1

In the thioglycollate test tube B, what kind of organism does the growth represent?

Select one of the following:

Microaerophile
Obligate/Strict aerobe
Facultative anaerobe
Obligate anaerobe

Explanation

Question 107 of 127

1

In the thioglycollate test tube C, what kind of organism does the growth represent?

Select one of the following:

Microaerophile
Obligate/strict aerobe
Facultative anaerobe
Obligate/strict anaerobe

Explanation

Question 108 of 127

1

In the thioglycollate test tube D, what kind of organism does the growth represent?

Select one of the following:

Microaerophile
Obligate/strict aerobe
Facultative anaerobe
Obligate/strict anaerobe

Explanation

Question 109 of 127

1

On a luria plate with Ampicillin, why is there no E. coli growth?

Select one of the following:

Because E.coli are sensitive to Ampicillin
Because E. coli are resistant to Ampicillin
Because luria does not contain the nutrients necessary for growth.

Explanation

Question 110 of 127

1

What protein allowed E. coli to grow on the plate with luria, Ampicillin and pGlo?

Select one of the following:

Beta lactamase
BLA
GFP
Green fluorescent protein

Explanation

Question 111 of 127

1

What gene allowed the E. coli to grow on the plate with luria, Ampicillin and pGlo?

Select one of the following:

BLA
Beta lactamase
GFP
Green fluorescent protein

Explanation

Question 112 of 127

1

What molecule allowed the E. coli to glow like a jellyfish?

Select one of the following:

Arabinose
Beta lactamase
Ara-C
Green fluorescent protein

Explanation

Question 113 of 127

1

What media is used for the antibiotic sensitivity test?

Select one of the following:

Mueller hinton
Kirby Bauer
Luria

Explanation

Question 114 of 127

1

What is the result of the beta lactamase test pictured?

Select one of the following:

It is pink and therefore, the test is negative and the microbe is sensitive to penicillin
it is pink and therefore, the test is positive and the microbe is sensitive to penicillin
it is pink and therefore, the test is positive and the microbe is resistant to penicillin
It is pink and therefore, the test is negative and the microbe is resistant to penicillin

Explanation

Question 115 of 127

1

Beta lactamase test

Select one of the following:

It is yellow and therefore, the test is positive and the microbe is resistant to penicillin
It is yellow and therefore, the test is negative and the microbe is resistant to penicillin
It is yellow and therefore, the test is positive and the microbe is sensitive to penicillin
It is yellow and therefore, the test is negative and the microbe is sensitive to penicillin

Explanation

Question 116 of 127

1

Why us thioglycollate a semi-solid agar?

Select one of the following:

Has agar in it and contains a reducing agent that produces water
Has agar and a reducing agent that produces CO2

Explanation

Question 117 of 127

1

Why are agar plates labeled on the bottom and inverted when incubated?

Select one of the following:

Labeled on the bottom in case you lose the lid and inverted during incubation to prevent condensation from dripping onto the agar surface
Labeled on the bottom in case you lose the lid and inverted so you can read the label

Explanation

Question 118 of 127

1

Why hold the test tube with your little finger while taking your bacterial sample?

Select one of the following:

So you do not contaminate the cap.
So the cap doesn't roll away.
So you can stretch your fingers.

Explanation

Question 119 of 127

1

Describe the proteins encoded in the pGLO plasmid.

Select one or more of the following:

Green fluorescent protein
Beta lactamase
Ara-C represser protein
Beta green protein

Explanation

Question 120 of 127

1

What is the principal of the beta lactamase test?

Select one of the following:

Does the bacteria have the BLA gene and therefore produce beta lactamase
Does the bacteria have the BLA protein and therefore produce the beta lactamase gene

Explanation

Question 121 of 127

1

Explain the regulation mechanism of the Green Fluorescent Protein gene expression.

Select one of the following:

Arabinose has to move the Ara-C represser protein out of the way so GFP can be transcribed and translated.
GFP takes up the arabinose so Ara-C can be transcribed and translated.

Explanation

Question 122 of 127

1

Define reducing agent

Select one of the following:

Sodium thioglycollate is a reducing agent and it reduces by binding O2 and converting it to water
Sodium thioglycollate is a reducing agent that reduces by causing evaporation of the broth

Explanation

Question 123 of 127

1

What are the criteria used to describe a bacterial colony>

Select one or more of the following:

Surface
Elevation
Whole colony
Edge
Color

Explanation

Question 124 of 127

1

Locations of items in the lab

Select one or more of the following:

Near the front door: Fire extinguisher, Eye wash, Shower, Gloves
Back of room: Fume hood
Lab bench: Disinfectant, Autoclave bags, Paper towels
Near Sinks: Bleach, Dyes used for stains, Paper towels (above)
Fume hood: Broken glass disposal box (underneath), Autoclave bags
Front podium: Phone
Back: Test tube racks, MSDS notebooks (back corner),
Next to fridge: Dustpan and broom

Explanation

Question 125 of 127

1

Which items are disposed of in the autoclave trash?

Select one or more of the following:

Used glass test tubes
Used glass slides
Broken glass
Used latex gloves
Paper towels used for bench disinfection
Contaminated Petri plates

	Created by Elle Ashe about 10 years ago

MCC Microbiology Lab Practical 2

Microbiology Lab Practical

Microbiology Lab Practical

Question 1 of 127

What method of staining was used to create this slide?

Select one of the following:

Explanation

Question 2 of 127

What kind of staining process was used to make this slide?

Select one of the following:

Explanation

Question 3 of 127

What kind of microbes are present on this slide?

Select one of the following:

Explanation

Question 4 of 127

Where should your backpacks and purses be stored during the lab period?

Select one of the following:

Explanation

Question 5 of 127

What items should you wear to protect yourself in the lab.

Select one or more of the following:

Explanation

Question 6 of 127

When do you disinfect your lab table and where do you discard the used paper towels?

Select one or more of the following:

Explanation

Question 7 of 127

You are dying of thirst (hunger, for smoke, chapped lips, bad breath) during the lab period. What do you do to alleviate your problem?

Select one of the following:

Explanation

Question 8 of 127

You notice your lab partner has caught the lab manual on fire. What should you do?

Select one or more of the following:

Explanation

Question 9 of 127

Your lab partner has managed to spill microorganisms all over the lab table. What should be done to clean up the spill?

Select one or more of the following:

Explanation

Question 10 of 127

A student broke an empty glass test tube. What should be done to clean up the glass and where should the pieces of glass be disposed of?

Select one or more of the following:

Explanation

Question 11 of 127

The instructor tripped on a backpack on the floor knocking her safety goggles off and splashing HCl in her eyes. What should she do?

Select one or more of the following:

Explanation

Question 12 of 127

After surviving the lab period you remove your gloves by turning them inside out and then where do you toss them?

Select one or more of the following:

Explanation

Question 13 of 127

Choose the two major types of microscopes that have been developed and the difference between them

Select one or more of the following:

Explanation

Question 14 of 127

Which microscopes are compound microscopes?

Select one or more of the following:

Explanation

Question 15 of 127

The ability of the microscope to enlarge an object.

Select one of the following:

Explanation

Question 16 of 127

How clear your specimen will appear when viewed through the lenses.

Select one of the following:

Explanation

Question 17 of 127

How well you lens is able to bend light.

Select one of the following:

Explanation

Question 18 of 127

What components influence the resolving power of a microscope?

Select one or more of the following:

Explanation

Question 19 of 127

Concentrates the light beam and directs light through the specimen.

Select one of the following:

Explanation

Question 20 of 127

A) Has a high lipid content and thin layer of tecoic acid underneath and stains pink.
B) Has a low lipid content and thick layer containing tecoic acids and stains purple.