Olivia Muench
Quiz by , created more than 1 year ago

Final lab practical quiz...good luck!

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Olivia Muench
Created by Olivia Muench over 5 years ago
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WSU BIO 2200 Lab Practical

Question 1 of 19

1

Microbiology Lab is Biosafety Level 2

Select one of the following:

  • True
  • False

Explanation

Question 2 of 19

1

Select from the dropdown list to complete the text.

Bacteria are ( ubiquitous, formite, thick ), since they can be found all over and in every environment.

Explanation

Question 3 of 19

1

Select from the dropdown lists to complete the text.

Bacteria have a ( negative charge, positive charge ). Because of this basic dyes, with their ( positive charge, negative charge ), stain the ( bacteria, background ). Acidic dyes, with their ( negative charge, positive charge ), stain the ( background, bacteria ).

Explanation

Question 4 of 19

1

Examples of acidic dyes:

Select one or more of the following:

  • Eosin

  • Methylene Blue

  • Crystal Violet

  • Nigrosin

  • Safranin

  • Congo Red

  • Maneval's stain

Explanation

Question 5 of 19

1

Which microscope was used in the lab?

Select one of the following:

  • Dark-field microscope

  • Phase contrast microscope

  • Bright-field microscope

  • Flourescence microscope

Explanation

Question 6 of 19

1

Select from the dropdown lists to complete the text.

Limit of Resolution:
D = ( Wavelength, NA condensor, NA objective lens ) / (( Wavelength, NA condensor, NA objective lens ) + ( Wavelength, NA condensor, NA objective lens ))

Explanation

Question 7 of 19

1

Smears should:

Select one or more of the following:

  • withstand washing

  • cover the entire slide

  • not be too thick

  • not result in excessive distortion of the organism

  • fall off the slide

Explanation

Question 8 of 19

1

Purpose of heat-fixing

Select one or more of the following:

  • kill bacteria

  • kill you

  • fix the bacteria to the slide

  • make cells more visible by coagulating cytoplasmic proteins

Explanation

Question 9 of 19

1

Pure cultures should have many different kinds of bacteria

Select one of the following:

  • True
  • False

Explanation

Question 10 of 19

1

Klebsiella Pneumoniae has a capsule

Select one of the following:

  • True
  • False

Explanation

Question 11 of 19

1

Choose what best describes capsules

Select one or more of the following:

  • Made of a polysaccharide layer called glycocalyx

  • Water soluble

  • Fat soluble

  • Heat sensitive

  • Prevents dehydration/dessication

  • Decreases virulence by protecting the cell from phagocytosis

  • Helps attachment to host cell

Explanation

Question 12 of 19

1

Gram-positive bacteria

Select one of the following:

  • Have an outer membrane/lipopolysaccharide layer (LPS)

  • Cell wall is ~90% peptidoglycan and has teichoic acid

  • Cell wall is ~10% peptidoglycan

  • are stained pink/red with safranin

Explanation

Question 13 of 19

1

Order of Gram staining:
1. with
2. with
3. with
4. with

Drag and drop to complete the text.

    Primary stain
    Crystal Violet
    Mordant
    Gram's Iodine
    Decolorizer
    95% Ethanol
    Counterstain
    Safranin

Explanation

Question 14 of 19

1

Which describes chemotaxis?

Select one of the following:

  • organism vibrates due to molecules hitting the cell

  • directional; independent movement over greater distances

  • movement towards chemical attractants and away from repellants

Explanation

Question 15 of 19

1

Which describes lophotrichous flagella?

Select one of the following:

  • flagella on both ends

  • flagella around perimeter of bacteria

  • a single flagella at one end

  • a tuft of flagella at one end

Explanation

Question 16 of 19

1

The motility test media is 0.4-0.5% agar and contains TTC (2,3,5-triphenyltetrazolium chloride)

Select one of the following:

  • True
  • False

Explanation

Question 17 of 19

1

Disadvantages of the motility test:

Select one or more of the following:

  • can be used to test for motility of pathogenic organisms

  • requires incubation time

  • if not performed properly, non-motile organisms appear motile

Explanation

Question 18 of 19

1

Fill the blank spaces to complete the text.

TTC is colorless when and red when .

Explanation

Question 19 of 19

1

Escherichia coli is motile and Staphylococcus epidermidis is non-motile.

Select one of the following:

  • True
  • False

Explanation

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