gina_evans0312
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Structural Basis for Biological Function (Protein Translocation) Quiz on E.Coli Secretion, created by gina_evans0312 on 21/12/2013.

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E.Coli Secretion

E.Coli Secretion

Question 1 of 13

1

What is the main problem of protein translocation?

Select one or more of the following:

  • Hydrophillic proteins must transverse two hydrophobic membranes and a hydrophillic space

  • Proteins must be unfolded and refolded again

  • Proteins must pass through an extremely crowded cytoplasm

Explanation

Question 2 of 13

1

Proteins to be secreted directly after translation have a peptidase sequence at the C-terminus

Select one of the following:

  • True
  • False

Explanation

Question 3 of 13

1

Which of the following parts of the peptidase sequence are closest to the N-terminus?

Select one of the following:

  • The basic residues

  • The hydrophobic region

  • Peptidase cleavage recognition site

Explanation

Question 4 of 13

1

There are 1-2 basic residues in the signal sequence, but the hydrophobic sequence is variable in length

Select one of the following:

  • True
  • False

Explanation

Question 5 of 13

1

When a protein is transcribed with the Peptidase signal site, what protein binds to its hydrophobic region?

Select one of the following:

  • Signal Recognition Protein

  • Ftsy- a GTPase

  • SecYEG- a bacterial pore

Explanation

Question 6 of 13

1

Ftsy transfers the protein into the pore

Select one of the following:

  • True
  • False

Explanation

Question 7 of 13

1

Which of the following provide the driving force for protein translocation across the membrane

Select one or more of the following:

  • Protonmotive force

  • FtsY's GTPase

  • Protein translation

Explanation

Question 8 of 13

1

What binds to the nascent polypeptide if Post-Translational Translocation takes place?

Select one or more of the following:

  • SecB

  • SecA

  • SecC

Explanation

Question 9 of 13

1

Post-Translational Translocation sequences have a string of hydrophobic amino acids, same as in Translational Translocation

Select one of the following:

  • True
  • False

Explanation

Question 10 of 13

1

What is the role of Sec_ ?

Select one of the following:

  • To keep the protein in solution till it binds to SecA at the pore

  • To fold the protein on its way to the periplasmic more

  • To stop the protein folding on its way to SecC

Explanation

Question 11 of 13

1

What forces are required to get a Post-Translational Translocation through the pore?

Select one or more of the following:

  • GTPase of FtsY

  • ATP hydrolysis by SecB

  • Protonmotive Force

Explanation

Question 12 of 13

1

Once inside the required membrane, what protein removes the peptide sequence?

Select one of the following:

  • Signal peptidase

  • Signal lyase

  • Signal Isomerase

Explanation

Question 13 of 13

1

The protein only folds after the signal sequence is cleaved

Select one of the following:

  • True
  • False

Explanation

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