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What does DNA synthesis requires?
Function of a DNA polymerase I?
DNA replication (chain, nucleotides, bonds)
Function of the 5 known polymerases?
What are the general requirements for all DNA polymerases?
Name the 2 types of polymerases and their function
How does proofreading work?
Mutagen
What are the three steps of polymerization?
Characteristic of PROKARYOTIC replication?
INITIATION step during prokaryotic replication?
What is the replicator sequence of E. coli?
Difference between a primer and a template
What is the problem one encounters during the elongation problem?
What are the basis for a semidiscontinous replication?
General characteristics of Leading strand
General characteristics of lagging strand synthesis
Okazaki fragments
Explain, in general, how does the elongation of the lagging strand works?
Explain replication in Eukaryotes base on E. coli
Why is it elongation in Eukaryotes more complicated?
Describe what is a replicon/replication unit
Why does the E. coli takes less time to replicate its genome than the humans
What happens at the ORC (Origin Recognition Complex) during the initiation of replication in eukaryotes?
Identify and explain the most common polymerases used during elongation in Eukaryotes
Briefly explain what are the Telomeres
What is a TelomerASE
Why is it important to have telomerase?
Definition of a genomec
Compare Euk. and Prok. Genomes
Technique used by DNA to fit the long stands
DNA packing in prokaryote
DNA packing in Eukaryote
Chromatin
Histones
Nucleosome
What are the characteristics of having a chromosome scaffold
Difference between transcription and translation
Difference between DNA and RNA
Difference between RNA and DNA synthesis
Identify and define the 4 main types of RNA
Function of Gene regulatory elements
Function of RNA polymerases
In what direction is RNA synthesized and how are DNA template read
What is the contemplate strand?
Explain the transcription in prokaryotes
Initiation process in Prokaryotes
Elongation process in Prokaryotes
Termination process in Prokaryotes
difference between introns and exon
Differences in the Eukaryotic Genome (introns and Exon)
Characteristics in Eukaryotes
Transcription factors?
Spliceosome?
RNA editing
General differences between Eukaryotes and Prokaryotes
