Method:

Place test tube 2cm3 1M HCl into 60oC waterbath.
Cut off 1-2cm root tip garlic root.
Put in watch glass contain 2cm3 acetic alcohol for 12mins.
Remove then place into another watch glass contain 5cm3 ice cold distilled water.
Leave for 4-5mins, then remove & dry.
Place tips into heated HCl for 5mins then repeat process again by step 3.
When tips fragile, transfer 1 tip to microscope slide, cut 4-5mm from growing tip (site of mitosis) & keep tip.
Gently macerate w/ mount needle, add 1 small drop of orcein ethanoic stain & leave 2mins.
Add coverslip & blot with filter paper.
View under microscope & identify stages of mitosis.

	Created by AD_ almost 10 years ago

AS Level AS Biology (IAL AS Biology Unit 3 ) Note on Observing Mitosis, created by AD_ on 23/04/2014.

Observing Mitosis