Question 1
Question
Mark the common feature between eukaryotes and prokaryotes
Question 2
Question
The largest bacteria which can be visible by unaided eye is_______
Question 3
Question
Which of the following is NOT bacteria?
Answer
-
Gram-positive bacteria
-
Mitochondrion
-
Chloroplast
-
Methanogens
-
Hyperthermophiles
Question 4
Answer
-
Bacteria and gives energy to cell
-
Archaea and gives energy to cell
-
Bacteria and gives strength to structure
-
Archaea and gives strength to structure
-
Bacteria and assists with growth
Question 5
Question
Which of the following is NOT the basic shape of the bacteria?
Answer
-
Rod shape
-
Square shape
-
Comma shape
-
Spiral shape
Question 6
Question
Which of the following organisms have thick peptidoglycan in their cell wall?
Answer
-
Gram-negative bacteria
-
Gram-positive bacteria
-
Yeast
-
Moulds
-
Prion
Question 7
Question
Each of the following statements concerning the Gram stain is correct except:
Answer
-
Escherichia coli stains pink because it has a thin peptidoglycan layer
-
Streptococcus pyogens stains blue because it has a thick peptidoglycan layer
-
Mycoplasma pneumoniae is not visible in the Gram’s stain because it does not have a cell wall
-
Mycobacterium tuberculosis stains blue because it has a thick lipid layer
-
Escherichia coli stains pink because it has a thick peptidoglycan layer
Question 8
Question
Which among the following are produced by microorganisms?
Answer
-
Fermented dairy products
-
Breads
-
Alcoholic beverages
-
All of the above
Question 9
Question
Type strain is used for referring to?
Answer
-
Species
-
Genus
-
Family
-
Division
Question 10
Question
The correct order of taxonomic groups from higher to lower rank is?
Answer
-
Kingdom—Order—Class—Family
-
Order—Class—Division—Family—Genus—Species
-
Kingdom—Order—Division—Family—Class—Genus—Species
-
Kingdom—Division—Class—Order—Family—Genus—Species
-
Order—genus—Class—Family—Division—Kingdom
Question 11
Question
What are ribosomes composed of?
Answer
-
Proteins
-
Proteins and DNA
-
DNA
-
RNA
-
Proteins and RNA
Question 12
Question
What is the correct order of staining reagents in Gram-Staining?
Answer
-
Crystal violet, alcohol, iodine solution, safranin
-
Crystal violet, iodine solution, safranin, alcohol
-
Crystal violet, iodine solution, alcohol, safranin
-
Crystal violet, safranin, alcohol, iodine solution
-
Iodine solution, crystal violet, alcohol, safranin
Question 13
Question
Which bacteria appears purple-violet colour after staining?
Question 14
Question
Which of the following are true for Gram-negative bacteria?
Answer
-
Upon alcohol treatment, the permeability of the cell wall increases
-
Crystal violet-iodine (CV-I) complex is extracted
-
Pore size decreases and the CV-I complex cannot be extracted
-
Alcohol treatment increases the permeability of the cell wall and the CV-I complex can be extracted
-
Upon alcohol treatment, the permeability of the cell wall decreases
Question 15
Question
Which of the staining technique helps in demonstrating spore structure in bacteria as well as free spores?
Answer
-
Acid-fast stain
-
Endospore stain
-
Capsule stain
-
Flagella stain
-
Blood stain
Question 16
Question
In Gram-staining, iodine is used as a______________
Answer
-
Fixative
-
Mordant
-
Solubilizer
-
unsolubilizer
-
Stain
Question 17
Question
Which of the following is the example of Gram-negative bacteria?
Answer
-
Lactobacillus
-
Eschericia coli
-
Staphylococcus aureus
-
Bacillus subtilis
Question 18
Question
Peptidoglycan layer is present in large quantity in?
Answer
-
Algae
-
Fungi
-
Gram-negative bacteria
-
Gram-positive bacteria
-
Archaea
Question 19
Question
The outer membarane of the Gram-negative cell wall is anchored to the underlying peptidoglycan by means of which of the following?
Answer
-
Braun’s Lipoprotein
-
Sugars
-
Phospholipids
-
Lipopolysaccharide
-
Proteins
Question 20
Question
The organisms which can use reduced inorganic compounds as electron donors are known
as _________
Answer
-
Chemotrophs
-
Euryarchaeota
-
Organotrophs
-
Lithotrophs
-
Phototrophs
Question 21
Question
The organisms which obtain energy by the oxidation of electron donor
are ________
Answer
-
Eukaryotes
-
Lichen
-
Crenarchaeota
-
Virus
-
Chemotrophs
Question 22
Question
Autotrophs are consumers. E.g. dogs, birds, fish and humans.
Question 23
Question
Halophiles thrive in high salt conditions
Question 24
Question
Psychrophiles thrive in high temperatures.
Question 25
Question
What is the conversion of carbon-containing molecules and nutrients into organic matter and uses oxidation of inorganic compounds as a source of energy?
Answer
-
Endocytosis
-
Phagocytosis
-
Protozoa
-
Binary Fission
-
Chemosythesis
Question 26
Question
Archaea are prokaryotes.
Question 27
Question
Which of the following are functions of water in the culture medium?
Answer
-
Nutrients must be in aqueous solution
-
Stable pH level
-
Cofactor of enzymes
-
Provides resistance to sudden transient temperature changes
-
It is a chemical reactant, nutrients must also be present in aqueous solution and provides resistance to sudden temperature changes.
Question 28
Question
Which of the following instrument is used for sterilizing the media after it has been prepared?
Answer
-
Autoclave
-
Distilled water
-
Laminar Air Flow Chamber
-
Inoculum Needle
-
Incubator
Question 29
Question
Colony formation can be observed in liquid media broth.
Question 30
Question
The temperature that allows for most rapid growth during a short period of time is known as __________.
Answer
-
Minimum Temperature
-
Optimum Temperature
-
Medium Temperature
-
Growth Temperature
-
Maximum Temperature
Question 31
Question
Mesophiles are group of bacteria that grow within the temperature range of?
Question 32
Question
The bacterium Staphylococcus aureus is which type of bacteria?
Question 33
Question
Microaerophilic bacteria require low levels of oxygen for growth.
Question 34
Question
Which of the following is a function of phosphates used in the preparation of media?
Question 35
Question
The optimum pH for the growth of most bacteria lies between_______
Answer
-
5 - 9
-
6.5 - 7.5
-
2 - 3.5
-
9 - 9.5
-
4 - 5
Question 36
Question
Growth of bacteria or microorganisms refer to __________________.
Answer
-
Glucose cello-logy
-
An increase in the size of an individual organism
-
An increase in the mass of an individual organism
-
Changes in the total population
-
An increase in number of cells
Question 37
Question
Transverse binary fission requires the formation of a crosswall(septum).
Question 38
Question
The synthesis of new membrane material during reproduction in Gram-positive cells is performed by which of the following organelles?
Answer
-
Nucleus
-
Mesosome
-
Endoplasmic Reticulum
-
Cytoplasmic membrane
-
Ribosome
Question 39
Question
When septum formation occurs near the pole of cell then it results in the formation of daughter cell known as _________
Answer
-
Microcell
-
Macrocell
-
Minicell
-
Daughter cell
-
New cell
Question 40
Question
Which of the following does not occur during binary fission in bacteria?
Answer
-
Chromosome separation
-
Cell elongation
-
Cytokinesis
-
DNA duplication
-
Spindle formation
Question 41
Question
The portion of the growth curve where a rapid growth of bacteria is observed is known as ____________
Answer
-
Lag phase
-
Logarithmic phase
-
Stationary phase
-
Decline phase
-
Improvement phase
Question 42
Question
Lag phase is also known as ___________
Question 43
Question
In which of the following phase secondary metabolites are produced during growth?
Answer
-
Lag phase
-
Log/Exponential phase
-
Stationary phase
-
Death phase
Question 44
Question
Which of the following is an indirect method for measuring bacterial growth?
Question 45
Question
Isolation of pure culture refers to ___________
Answer
-
Purification of culture
-
Trapping all microorganisms
-
Introduction of inoculum
-
Separation of a single colony
-
To grow microorganisms on a surface
Question 46
Question
Enrichment media allows the growth of a large number of varied bacterial species
Question 47
Question
A large cluster of colonies are obtained in streak plate method.
Question 48
Question
The respiratory chain of bacteria is associated with the _____________
Answer
-
Mitochondrial membrane
-
Cytoplasmic membrane
-
Cell wall
-
Cytoplasm
-
Ribosome
Question 49
Question
Safranin will stain bacterial cells blue
Question 50
Question
Cocci is spherical shaped.
Question 51
Question
Protozoa is colourless
Question 52
Question
What does protozoa use to obtain nutrients?
Answer
-
Osmosis
-
Photosynthesis
-
Chemosynthesis
-
Phagocytosis
-
Passive transport
Question 53
Question
Fungi have a cell wall
Question 54
Question
Viruses of bacteria that can kill and lyse the bacteria they infect are called _________
Answer
-
Lichen
-
Infections
-
Vaccination
-
Anti-microphile
-
Bacteriophage
Question 55
Question
Protozoa are eukaryotes
Question 56
Question
Whats made up of single types of protein subunits stacked around a central axis. (E.g. Tobacco mosaic virus.)
Answer
-
Polyhedral
-
Helical
-
Complex
-
Envelop
-
Spherical
Question 57
Question
What virus has a tail?
Answer
-
Helical
-
Polyhedral
-
Complex
-
Envelop
-
Spherical
Question 58
Question
Adenovirus is an _______
Answer
-
Helical
-
Polyhedral
-
Complex
-
Envelop
-
Spherical
Question 59
Question
Viruses are unicellular
Question 60
Question
Protein synthesis occurs in the Influenza life cycle.
Question 61
Answer
-
pH 5 or below
-
pH 6 - 8
-
pH 9+
Question 62
Question
What cannot grow in the presence of oxygen
Answer
-
Strict aerobe
-
Strict anaerobe
-
Facultative anaerobes
-
Aero-tolerent anaerobes
-
Micro-aerophile
Question 63
Question
What grows better in the presence of oxygen, but will grow without it?
Answer
-
Strict aerobe
-
Strict anaerobe
-
Facultative anaerobes
-
Aero-Tolerant anaerobes
-
Micro-aerophile
Question 64
Question
What depends on oxygen to grow
Answer
-
Strict aerobe
-
Strict anaerobe
-
Facultative anaerobes
-
Aero-Tolerant anaerobes
-
Micro-aerophile
Question 65
Question
Gram negative bacteria......
Which statement is wrong?
Answer
-
10% of cell wall is peptidoglycan
-
Contains teichoic acid
-
No outer membrane
-
Does not retain crystal violet
-
High lipid/lipoprotein content
Question 66
Question
Gram postive bacteria....
Which answer is wrong?
Question 67
Question
The function of bacteria capsule is to _________
Answer
-
Link amino acids together in the order specified by mRNA.
-
Assit in attaching to other cells and surfaces.
-
Help bacteria move towards nutrients and away from toxic chemicals.
-
Keep bacterium from drying out and protects from phagocytosis.
-
Regulate passage of substances in and out of cell.
Question 68
Question
The function of bacteria pilli is to _________
Answer
-
Link amino acids together in the order specified by mRNA.
-
Assit in attaching to other cells and surfaces.
-
Help bacteria move towards nutrients and away from toxic chemicals.
-
Keep bacterium from drying out and protects from phagocytosis.
-
Regulate passage of substances in and out of cell.
Question 69
Question
Bacterial life cycle is an asexual process
Question 70
Question
Lysozymes in tears kills ________.
Answer
-
Infection
-
Viruses
-
Moulds
-
Gram-positive bacteria
-
Gram-negative bacteria
Question 71
Question
Influenze virus is polyhedral
Question 72
Question
Adenovirus is polyhedral.
Question 73
Question
Bacteria from a pure culture which is used to start a new culture is called __________?
Answer
-
Spreading
-
Agar
-
Gram negative
-
Gram postive
-
Inoculum
Question 74
Question
Enveloped viruses are less sensitive to drying/heating conditions rather than naked capsid viruses.
Question 75
Question
The naked capsid virus is more hostile compared to the enveloped virus due to it breaking down the cell membrane through lysis.
Question 76
Question
In the virus life cycle, an active, intracellular stage is called the __________ stage.
Answer
-
Metabolic
-
Infectious
-
Log
-
Attachment
-
Biosynthesis
Question 77
Question
The area of no growth is what we call a ____________
Answer
-
Viral plaque
-
Complex
-
Plaque assay
-
Inoculum
-
Spec
Question 78
Question
Where would you find a prophage?
Answer
-
Virus life cycle
-
Lytic life cycle
-
Lysogenic life cycle
-
Bacterial life cycle
-
Food life cycle
Question 79
Question
Another word for lytic is [blank_start]virulent[blank_end]
Question 80
Question
Another word for lysogenic is [blank_start]temperate[blank_end].