456291
Descripción
Test por gina_evans0312, actualizado hace más de 1 año
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Creado por gina_evans0312
hace más de 10 años
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Pregunta 1
Pregunta
Gene synthesis can be fully automated cheaply
Respuesta
- True
- False
Pregunta 2
Pregunta
Why is genome synthesis difficult?
Respuesta
-
Chemical synthesis is limited to 100nt at a time
-
Manipulation of large fragments is difficult
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Vectors are limited
Pregunta 3
Pregunta
Synthesis of a genome must be done in small, overlapping parts
Respuesta
- True
- False
Pregunta 4
Pregunta
How many kbpairs can PCR be used for?
Respuesta
-
1-5
-
5-10
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10-15
Pregunta 5
Pregunta
What is essential when creating small fragments to be joined together later in clones?
Respuesta
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The fragments must overlap
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The fragments MUST be 7kb long
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The fragments cannot contain introns
Pregunta 6
Pregunta
When transplacting the synthetic genome between clones, what must not be present?
Respuesta
-
Proteins
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DNA
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RNA
Pregunta 7
Pregunta
How is the receptor organism for the synthetic genome made able to receive it?
Respuesta
-
The cell wall is treated with chemicals to make it permeable
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The DNA is directly microinjected into the cell
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The cell absorbs the DNA with other nutrients
Pregunta 8
Pregunta
Once the synthetic genome has been added to the cell, it divides as it contains two genomes
Respuesta
- True
- False
Pregunta 9
Pregunta
Why synthetic organisms?
Respuesta
-
To see if we can
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To improve/redesign regulatory networks
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To produce protective genes
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Biofuels/biopharmecuticals
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