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Culturing Microorganisms

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GCSE CGP Biology Mapa Mental sobre Culturing Microorganisms, creado por Z S el 07/01/2017.
Z S
Mapa Mental por Z S, actualizado hace más de 1 año
Z S
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Culturing Microorganisms
  1. You can grow bacteria in the lab
    1. Bacteria are cultured in a culture medium
      1. This contains the carbohydrates, minerals, proteins and vitamins they need to grow
        1. Can be a nutrient broth solution or solid agar jelly
          1. Bacteria grown on agar plates will form visible colonies on the surface of the jelly, or will spread out to give an even covering of bacteria
            1. To make an agar plate, hot agar jelly is poured into shallow round plastic dishes called Petri dishes
              1. When the jelly's cooled and set, inoculating loops can be used to transfer microorganisms to the culture medium
                1. The microorganisms then multiply
        2. In the lab at school, cultures of microorganisms are not kept above 25 C
          1. Because harmful pathogens are more likely to grow above this temperature
          2. In industrial conditions, cultures are incubated at higher temperatures so that they can grow a lot faster
          3. You need to use uncontaminated cultures
            1. Contamination by unwanted microorganisms will affect the results
              1. It can also potentially result in the growth of pathogens
              2. The Petri dishes and culture medium must be sterilised before use to kill any unwanted microorganisms that may be lurking on them
                1. If an inoculating loop is used to transfer the bacteria to the culture medium, it should be sterilised first by passing it through a hot flame
                  1. After transferring the bacteria, the lid of the Petri dish should be lightly taped on
                    1. To stop microorganisms from the air getting in
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