Clone Identification and Expression

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A radioactive probe can take what forms?

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Probes can be

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Name the two ways probes can be designed

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What is codon bias?

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80% of Phe codons are TTC, 20% are TTT

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If the protein is an enzyme it can be found by adding the substrate

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State the possible forms of Ig's for protein separation?

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Probes needn't be 100% accurate to work

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For a Western Blot, how is the DNA stuck to the membrane?

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Once the DNA has been bound, what happens next?

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In an expression vector, where is the new gene added?

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Name the parts on the vector

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More compliated vectors have proteins tagged for purification

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What is involved in His Chain Purification?

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His Purification uses Histadine's affinity for what metal?

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His-tagged proteins will bind to Nickel in a column. Others that don't bind will be washed off.

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The PET system involves using a promoter for the protein that you can control the expression of

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What promoter is used in the PET system to control protein expression?

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In the PET system, what promoter is used to control the T_ expression?

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What is required for a good shuttle vector?

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Shuttle vectors are usually plasmids with additional sequences

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Shuttle vectors for yeast/fungi need

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A baclovirus would be used as a vector into what?

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Secretion of certain proteins can be caused by what?

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Why are there two origins in the Yeast shuttle vector?

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What is the method of selection in yeast?

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Like bacteria, yeast cannot take up linear DNA

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What does a YAC contain that a plasmid does not?

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Yeast can allow DNA to be directly inserted into its genome

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How can yeast accept DNA into its genome?

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In order to directly insert a gene, you must flank the gene with identical stretches of the part of the genome you want to insert it in

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Plants would use an aglobacterium T1 plasmid

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How do you replicate transformed plant cells?

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Mammal shuttle vectors are the same as yeast vectors, but the yeast origin is replaced with what?

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Retroviruses can also be used to insert transgenes into mammilian genomes by packing the transgene in a retroviral capsule

Year 2 Test sobre Clone Identification and Expression, creado por gina_evans0312 el 29/12/2013.

Clone Identification and Expression

A radioactive probe can take what forms?

Probes can be

Name the two ways probes can be designed

What is codon bias?

80% of Phe codons are TTC, 20% are TTT

If the protein is an enzyme it can be found by adding the substrate

State the possible forms of Ig's for protein separation?

Probes needn't be 100% accurate to work

For a Western Blot, how is the DNA stuck to the membrane?

Once the DNA has been bound, what happens next?

In an expression vector, where is the new gene added?

Name the parts on the vector

More compliated vectors have proteins tagged for purification

What is involved in His Chain Purification?

His Purification uses Histadine's affinity for what metal?

His-tagged proteins will bind to Nickel in a column. Others that don't bind will be washed off.

The PET system involves using a promoter for the protein that you can control the expression of

What promoter is used in the PET system to control protein expression?

In the PET system, what promoter is used to control the T_ expression?

What is required for a good shuttle vector?