objectives

protein-cell interactions

cell functions

cell types

cellular structure

Cell Plasma Membrane

Phospholipids

Lipid bilayer

Transmembrane Proteins

Selectively permeable
(depends on...)

"outside-in" signalling

"inside-out" signalling

Protein-based Receptors

Adhesion receptors

Common Receptor Molecules

Cell Adhesion Force

Types of cell contacts

Cell-cell interactions
(3)

Cell-ECM

Cadherins

Integrins

Cell surface interactions

Cell surface receptors

Extracellular Matrix
(ECM)

ECM types

Ex. 6.1
proteins often adsorb to biomaterial surfaces implanted in vivo or exposed to serum-containing media in vitro.

Many biomaterials do notsupport cell adhesion prior to adsorption of a protein layer. How might proteins facilitate adhesion of cells to a biomaterial?

Hint: consider to coat ECM proteins of biomaterial surface.

basic interactions

Cell spreading

in vivo
vs.
in vitro

Types of cell cultures

characterization methods

upright vs inverted microscope

bright field vs dark field microscope

Phase-Contrast microscope

Fluorescence microscopy

Fluorescence microscopy sample preparation

Confocal fluorescence microscopy

confocal fluorescence microscopy
common charactersistics

Cell Adhesion Assays

Cell Spreading Assays

Centrifugation Assay (Normal Force)

Flow Chamber Assay (Shear Force)

Cell Migration Assay

flow chamber vs centrifuge