Gen. Lec. Notes 10/20-10/23 (Chapter 10)

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DNA as the genetic material, part 2.
Isabella N
Flashcards by Isabella N, updated more than 1 year ago
Isabella N
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Question Answer
DNA is short for? Deoxyribose acid
DNA is shaped as a_____, made of ____? pentose; sugar
Pyrimidines + purines are? organic nitrogenous bases
RNA is short for? Ribonucleic acid
Pyrimidines are ___ rings. Single
Name the (3) pyrimidines Cytosine, thymine, uracil
Purines are ___ rings. Double
Name the (2) purines Guanine, adenine
How is nucleoside made? A base bound by its OH to the H of a pyrimidine/purine
A nucleoside has what (2) things? A nitrogenous base + pentose sugar
A nucleotide has what? A phosphate + nucleoside
_____ are the basic building blocks to ____. Nucleotides; DNA
On a nucleotide, the ______ groups is on _-_' position? Phosphate; C-5'
What is difference between nucleoside DIphosphate + nucleoside TRIphosphate? Diphosphate = (2) phosphates, trisphosphate = (3) phosphates
DNA is a ____ molecule. Polar
Nucleotides' phosphate groups are linked by _____ bonds, always directionally linked _' to _'. phosphodiester; 3, 5
The __ group is alway on the _' carbon. OH; 3
Chargaff's rule/equation (A+T) = (C+G)
Who's rule states that the % of (C+G) =/= % of (A+T)? Chargaff's rule
______ + _____ discovered the ______ _____ model using Franklin's X-ray pic. Watson, Crick; 2x helix
Watson + Crick knew what (2) things about DNA? It has a helical structure + has (2) polynucleotide strands
DNA strands are __________ + bases are ______ on ___ of each other. antiparellel; stacked, top
Base pairings are...? A—T + G—C
There are how many base pairs per helix turn? 10
A-T has how many H-bonds between each other? 2
G-C has how many H-bonds between each other? 3
How is the DNA structure stabilized? The arrangements of base pairings
Separation of DNA is called...? Denaturation
Renaturation of DNA is called what? Hybridization
Hybridization of DNA is when? (2) complementary DNA strands join together
Hyperchromic shift is seen during? DNA denaturation/heating
How is hyperchromic shift measured and what does it determine? By the melting point of DNA; the base composition of DNA
The higher the _______ point of DNA, the higher base pairings of _—_. Melting; G,C
Separating H-bonds in DNA structure is _______. Irreversible
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