Merchant Reading (2007)

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The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions
Candice Young
Flashcards by Candice Young, updated more than 1 year ago
Candice Young
Created by Candice Young about 6 years ago
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Question Answer
Chlamydomonas reinhardtii unicellular green algae with mitochondria two anterior flagella for motility + mating one chloroplast grows in the dark on an organic carbon source while maintaining a functional photosynthetic apparatus
What scientific classification is Chlamydomonas reinhardtii? Chlorophyta = division Chlorophyceae = class Chlamydomonadales = order Chlamydomonadaceae = family Chlamydomonas = genus reinhardtii = species
What type of major division of algae are Chlamydomonas reinhardtii? Green algae
Which general genes of Chlamydomonas are shared with animals but have been lost in angiosperms? those encoding proteins of the eukaryotic flagellum (or cilium) and the associated basal body (or centriole)
how many chromosomes were found in Chlamydomonas? 17 (in the draft genome)
how are ribosomal RNA genes arranged in eukaryotes? arranged in tandem arrays
EST Expressed Sequence Tags --> used to create protein-coding gene predictions
What is strange about the introns of Chlamydomonas? they are longer than that of many eukaryotes many repeat sequences -> may be subject to creation/invasion by transposable elements!
What suggests extensive gene duplications in Chlamydomonas? Half of the genes of the two-gene families are arranged in tandem
What transporters were found in Chlamydomonas? membrane transporters: ion channels, ATP binding cassettes, some related to flagellar function (redistribute intracellular Ca2+), some substrate specific
What did this paper compare the Chlamydomonas proteome to? The human and Arabidopsis genome --> proteins typically more similar to Arabidopsis with exception in flagellum and basal body proteomes (found in animals but not angiosperms)
Where were more homologous proteins found between Chla. and humans and Arabidopsis? Between Chlamydomonas and Arabidopsis but not shared with humans
How might there be homologous proteins between Chlamydomonas and angiosperms that are missing from humans? 1) proteins were present in plant-animal ancestor and retained in Chlamydomonas + angiosperms, but lost in animals 2) horizontally gene transfer into Chlamydomonas 3) arose in the plant lineage after divergence of animals (but before the divergence of Chlamydomonas)
How did diatoms, brown algae, and chlorophyll a & c containing algae get their photosynthetic organelles? through SECONDARY endosymbiosis
How did green and red algae get their photosynthetic organelles? through PRIMARY endosymbiosis
What is the GreenCut? families that: contain Chlamydomonas proteins contain proteins from Ostreococcus, Arabidopsis and moss do NOT contain proteins from nonphotosynthetic organisms
What about PlantCut and Diatom Cut? PlantCut: compare GreenCut proteins to red alga C. merolae --> get a subset of proteins present across the plant kingdom DiatomCut: GreenCut protein families that also included representatives from the diatoms T. pseudonana or P. tricornutum
PlastidCut hypothesis --> protein families present in both the PlantCut and DiatomCut should contain only those GreenCut proteins associated with plastid function
How are reactive oxygen species produced by photosynthesis regulated in the cell? thioredoxins (found in plastids)
What is the eye spot in Chlamydomonas? a structure that senses light and triggers phototactic responses contains pigment granules and thykaloid membrane
AKCs required for eyespot assembly
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