Βacillus

Sotia P.
Flashcards by , created 4 months ago

Microbiology Flashcards on Βacillus, created by Sotia P. on 04/14/2019.

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Sotia P.
Created by Sotia P. 4 months ago
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Question Answer
What are the microscopical shape and size of Bacillus? large rods- 10μm motile
Name the 2 staining methods for bacillus and their results 1. gramm staining= + 2. wirtz-conclin= green endospores and the rest red.
Cultivation method for bacillus: * MPBA * 37°C for 1-2 days * Aerobes/facultative anaerobic
Which animals are the most susceptible to infection by B. Anthracis? Ruminants
Which form of B. Anthracis is the most common? The cutaneous form- 95%
Name 3 forms of B. Anthracis: 1) Cutaneous 2)Pulmonary 3) GIT form
Which bacillus causes a dangerous zoonotic disease? What is the name of the disease? B. Anthracis causes Anthrax, splenic fever
What are the results of Catalase and oxidase test for bacillus? Cat + Ox -
What is the name of the test that is used for detecting anthrax ? Ascoli reaction/ precipitation test Adding antigen on top of antiserum with Ab’s and the result is a ring in the middle
Which bacillus is positive for ascoli and perl test, and which is negative? + : B. Anthracis - : B. Cereus
Which strain causes hemolysis? What type? B. Cereus- β haemolysis
All strains of bacillus are motile Nope Only B. Cereus is (B. Anthracis is non-motile)
None of the strains have capsula B. Anthracis has a capsule
In order to prevent the spread of anthrax from dead bodies it is necessary to: Bury the carcasses deep down or keep it at 150°C for 3h. The cell wall of the bacteria breaks when heated.
What is typical for pulmonary form of B. Anthracis? Chest edema followed with cardiovascular shock. High mortality
High temperature and eschars are typical for: Cutaneous form of B. Anthracis Causes black necrotic lesions aka eschars
The less common form of B. Anthracis which however is fatal after ingesting the spores is: GIT form
** bonus question :) ** Which bacillus was used as a biological weapon for terrorist attacks and when?? B. Anthracis in 2001, USA office attacks
The toxins of B. anthracis in general cause: swelling, necrosis, septicemia all will lead to increased vascular permeability, hemorrhages and death.
Zinc metalloprotease is produced by____and causes____ : B. Anthracis death of macrophages
Which strain produces enterotoxins? What do they cause? B. Cereus Will cause alimentary intoxication, gastritis, enteritis, vomiting and diarrhea
Which strain possess a β-lactamase gene? What does this mean for the bacterium? B. Anthracis When the gene is activated the becterium will become resistan to β-lactam antibiotics making it more difficult to treat.
What test is used for detection of enterotoxins? ELISA
2 not so important strains of bacillus are: B. Subtillis- mastitis B. Larvae- plague of bees fetus
What is the process for wirtz-conclin staining? • Heat fixation on slide • 5% malachite green • Heat ~~Repeat green and heat 3 times~~ • Wash with water • Carbolfuchsin staining
What is one factor that gives the bacteria their increased resistance? Sporogenic: produce spores which give them increased resistance
What happens in splenic fever? The spleen is destroyed and there are problems with circulation
Is splenic fever transmissible? YES Usually from CATTLE to human from SKIN= cutaneous/skin transmissible. Causes sepsis (blood intoxication), increased temp., necrotic skin lesions
Which strain is opportunistic? B. Cereus Also survives heat (heat treatment in food) and so it's an important contaminant of food