The Mancini Technique (Radial Immunodiffusion)

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From the 04/10/13 lab.

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Question Answer
What is the type of diluting performed in the Mancini technique? Doubling dilution.
What type of technique is the Mancini technique and why? It is a quantitative technique, because a standard curve is produced by a series of dilutions of a standard solution, which allows the concentration of unknown samples to be determined.
Why is it important that the antibody (anti-IgG) is mixed well in the agar? The technique is based on antigen diffusion and a precipitation reaction. The antibody must be evenly spread throughout the agar or an accurate equivalence point cannot be found, and diameter of the precipitin ring produced will not be proportional to antigen concentration.
What were the concentrations of the dilutions used in the Mancini assay? 8, 4, 2, 1, and 0.5 mg/ml.
Which is more sensitive: immunoelectrophoresis or radial immunodiffusion? Why is this significant? Radial immunodiffusion is more sensitive - this is important because it is of more clinical use given that the quantities of antigen/antibody do not have to be strikingly different from normal to obtain an accurate result, and the technique is still accurate in small sample sizes.
In which three BROAD clinical situations might radial immunodiffusion be used? To look for: immunodeficiency, immunoproliferative disorders, or an appropriate immune response to a stimulus.
Complete the sentence with regard to the equivalence point: The more concentrated the antigen/antibody in the well... ...the further it will diffuse to reach its equivalence point, and the larger the diameter of the precipitin ring will be.
What is the normal range of serum IgG in a healthy patient? 7-16 mg/ml.
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