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3896336
MEDICIÓN POTENCIAL DE MEMBRANAS
Mind Map by
Valentina Sanchez Luna
, updated more than 1 year ago
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Created by
Valentina Sanchez Luna
over 5 years ago
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iblnjl
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tt
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Resource summary
MEDICIÓN POTENCIAL DE MEMBRANAS
1 OBJETIVO
1.1 Verificar
1.1.1 Permeabilidad de distintas membranas
1.1.2 Mediante potencial eléctrico
2 INTRODUCCIÓN
2.1 Membrana y Factores que afectan la permeabilidad
2.1.1 CÉLULAS
2.1.1.1 Membrana organelos
2.1.1.1.1 regula intercambio de materia celular.
2.1.1.1.2 Separan, aislan, contienen enzimas y metabolitos
2.1.2 MEMBRANA
2.1.2.1 Fosfolípidos
2.1.2.1.1 Anfipáticos poco solubles en agua
2.1.2.1.2 Forman bicapa
2.1.2.1.2.1 Barrera estable entre compartimientos acuosos.
2.1.2.1.2.1.1 Estructura básica de membranas biológicas.
2.1.2.2 Organismos multicelulares
2.1.2.2.1 Mantiene integridad de células
2.1.2.2.1.1 Coordina actividades
2.1.2.2.1.1.1 Establece condiciones de pH
2.2 Mecanismos de transporte por medio de membranas
2.2.1 DIFUSÓN
2.2.1.1 Partículas de medios de mayor concentración pasan a medios de menor concentración
2.2.2 ÓSMOSIS
2.2.2.1 Equilibrio entre concentraciones de agua
2.2.2.1.1 Conc. Isotónica
2.2.2.1.1.1 La cantidad que entra es igual a la que sale.
2.2.2.1.1.2 Conc. Hipertónica
2.2.2.1.1.2.1 Inicialmente hay mucha agua, luego se deshidrata
2.2.2.1.2 Conc. hipotónica
2.2.2.1.2.1 Se expande y ocurre lisis debido a mucha agua al interior
3 METODOLOGÍA
3.1 Tener en cuenta medidas de precaución para el manejo del KCl.
3.1.1 Montaje
3.1.1.1 Calibración pH-metro
3.1.1.1.1 Determinación de potencial eléctrico y pH
3.1.1.1.1.1 Contar tiempo, hacer cálculos y analizar.
4 MATERIALES
4.1 Solución de KCl
4.1.1 Vasos de precipitado
4.1.1.1 Bandas de caucho
4.1.1.1.1 Soporte universal
4.2 Aro con nuez
4.2.1 pH-metro
4.2.1.1 Vaso con agua destila
4.2.1.1.1 Cronómetro
4.2.1.1.1.1 Membrana semipermeable
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