463394
Description
Mind Map by jessica.lynn.wal, updated more than 1 year ago
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Created by jessica.lynn.wal
about 11 years ago
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Recombinant
DNA Technology
- Polylinkers
- Lambda Bacteriophages
- Cloning Longer Fragments
- BACs
- YACs
- BACs
- Protein Production for:
- Commercial/Therapeutic research
- Overproduction in new host
- Expression Vectors
- Commercial/Therapeutic research
- Recombinant proteins expression systems
- Baculovirus
- Cells do not survive infection
- Do not add all sugars
on human proteins
- infect invertebrate larvae
- Cells do not survive infection
- Bacteria
- Difficult to produce complex proteins
- Proteins can form aggregates
- Cannot always fold proteins
- Cannot cleave propoteins
- Lack post-translation modification mechanisms
- Difficult to produce complex proteins
- Yeast
- Cannot fold some proteins
- Cannot glycosylate without genetic engineering
- Did not add S-S to HBV vaccine
- Cannot fold some proteins
- Mammalian Cells
- Relatively low levels of production
- Gycosylation occurs
differently in different
cells
- Higher cost
- Relatively low levels of production
- Transgenic Animals
- Spider silk in goat milk
- Chickens
- Spider silk in goat milk
- Baculovirus
- Site Directed mutagenesis using PCR
- Use of tagged proteins
- fusion proteins
- fusion proteins
- Epitope Tags
- Tandem Affinity purification
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