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4991282
Light microscopes
Description
Light Microscopes - Advantages, disadvantages etc.
No tags specified
light microscope
microscope
biology
science
cell study
cells
biology unit 1
unit 1
as biology
studying cells
cell structure
gcse
Mind Map by
Nathan Coates
, updated more than 1 year ago
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Created by
Jessica Phillips
almost 8 years ago
Copied by
Nathan Coates
almost 8 years ago
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Resource summary
Light microscopes
Attachments:
Electron Microscopes
Advantages
No vacuum
Specimen can be dead or alive
Low cost
Good for use in schools etc.
Portable
Less likely to produce atifacts
Natural colours seen
Preparation of specimen
Less harsh than for EM
No technical expertise required
Easy to use
Disadvantages
Low magnification
Magnification - X1500 to X2000
Low resolution
Can't produce 3D images
Not as detailed as electron microscopes
Improvements to resolution
Having an objective lens with a short vocal length
Having a film of immersion oil between the objective lens and the specimen on the slide
Staining
Annotations:
Stains are coloured chemicals used to distinguish (highlight) specific structures.
The first known stain was saffron.
Coloured stains
Stains that bind to specific chemicals on or in the specimen allowing it to be seen
Differential stains
Involves more than one stain on a specimen
Used for contrast
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