UV-Visible Spectroscopy

Which block of the periodic table contains the metal ions that can absorb energy to promote electrons to the outer energy levels of the metal?

Other than emitting energy quanta that correspond to certain wavelengths of light, what other property can be a result of electrons returning to their original energy levels in D block metal ions?

What are conjugated bonds?

What are chromophores?

Why do conjugated bonds result in chromophore properties?

What word, used in Art too, describes the relationship between the visible wavelength at which energy is absorbed, and the visible wavelength that is produced?

What energy levels do long and short wavelengths of light have?

How are wavelength and energy related, demonstrated in Planck's Law: E = (h x c)/ λ ?

At which wavelengths does chlorophyll absorb?

What is the Beer-Lambert Law and what does it state?

What is molar absorptivity?

In what range are UV wavelengths?

What structural properties could an organic substance have if it absorbs UV light?

In a colorimeter for measuring the absorption of visible light by a substance, what provides the white light source that is diffracted?

In a colorimeter for measuring absorption of visible light by substances, what two pieces of equipment may be used to diffract the white light source?

What is used to ensure that only one wavelength of light is assayed at a time?

In a colorimeter for measuring absorption of visible wavelengths by substances, how is a beam of monochromatic light split so that one can be used for a reference cell and one for the sample?

What type of detector measures the transmission of wavelengths in a colorimeter to measure the absorption of visible light by substances?

How many nm is the limit at which your solvent should absorb within the region you're interested in?

What polar, saturated solvents could be used in UV-Visible spectroscopy?

In a colorimeter, why might you choose to use a glass cuvette rather than a plastic cuvette for your sample?

What is the disadvantage to using a single beam colorimeter and how might this be solved?

How might one conduct a quantitative analysis of a substance using a colorimeter?

Why might a concentration-absorption (calibration) graph be linear?

How might one carry out an analysis of an unknown biological specimen?

Why might your results be inaccurate/there be deviations from Beer's Law? How might this be minimised?

Why is Beer's Law not obeyed at high concentrations? How might this be resolved?