What is so special about cGMPPK?
The subunits of the dimer can swap roles of damage occurs
It's only found in cardiac cells
How many cGMP bind per monomer?
Which order do the subunits go in (from N to C terminus)
A= Dimerisation Domain
B- Catalytic Domain
C= Autoinhibitory Hinge Region
D= cGMP binding sites 1 & 2
What is the purpose of the autoinhibitory hinge region?
Acts as a pseudosubstrate
Causes inhibition after a certain amount of activity
What motif does cGMP Stimulated Protein Kinase Phosphorylate?
What is the role of this protein?
The activation of cGMP dependent ion channels
Activates cGMP dependent phosphodiesterases
The activation of cGMP dependent proteins
Inhibits cGMP dependent phosphodiesterases
What is required for NO to be synthesized?
Which of the following stimulate NO production?
A, B, D, F
A, D, E, F
B, C, D, F
Which system is NO part of?
NO is synthase a monomer- True or False?
How many types of NO synthase are there?
Constitutive & Inudcible
Why is NO produced by iNOS (Immune NO Synthase)
It kills of pathogens
It's used to signal immune cells
It's used to bind and immobalise pathogens
NO Synthase produces a few mmol of NO over a minute- True or False?
False- its pmol over a minute
False, it's micromol in a few seconds
False, its pmol over a few seconds
NO Synthase contains the following, but in which order?
A-NADPH binding site
B-Calmodulin Binding Site
D- FAD centre
F- Arg, O2 and Tetrahydropterin (Active site)
Why are vascular endothelial cells equipped to cause vasodilation?
It allows them to reduce blood pressure according to need
It allows them to increase blood flow to wounded areas
It prevents damage to veins under high pressure
Which neurotransmitter effects which cells?
Vascular Endothelial- A&B
Vascular Endothelial- A
What is the role of nitroglycerin?
It liberates NO from tissues
Causes an increase in NO synthesis
What is the role of viagra?
Prevents cGMP breakdown
Prolongs NO production