Chapter 5 Exploration of Genes Relies on Key tools

Question 1 of 25

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Advances in biotechnology are possible because of key techniques such as

Select one or more of the following:

  • Restriction enzyme analysis

  • Blotting techniques

  • DNA sequencing

  • solid- phase synthesis of amino acids

  • solid-phase synthesis of nucleic acids

  • PCR

Question 2 of 25

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Restriction enzymes split DNA into specific fragments

Select one of the following:

  • True
  • False

Question 3 of 25

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Restriction enzymes are

Select one of the following:

  • bacterial enzymes that cleave DNA in a highly specific manner

  • fungal enzymes that cleave DNA in a highly specific manner

  • phage enzymes that cleave DNA in a highly specific manner

Question 4 of 25

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The restriction enzymes recognize

Select one of the following:

  • cleavage sites of three to eight nucleotides in length that are palindromic and then cleave each strand of the DNA

  • cleavage sites of four to eight nucleotides in length that are palindromic and then cleave each strand of the DNA

  • cleavage sites of four to eight nucleotides in length that are non-palindromic and then cleave each strand of the DNA

  • cleavage sites of three to eight nucleotides in length that are non-palindromic and then cleave each strand of the DNA

Question 5 of 25

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DNA fragments can be separated by gel electrophoresis

Select one of the following:

  • True
  • False

Question 6 of 25

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In gel electrophoresis, fragments can be visualized by staining with

Select one of the following:

  • agarose

  • polyacrylamide gels

  • ethidium bromide

  • bromide

Question 7 of 25

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Separated fragments of DNA are transferred to a sheet of

Select one of the following:

  • cellulose paper, a process called southern blotting, and then exposed to a radioactively labeled probe complementary to the sequence of interest

  • nitrocellulose paper, a process called southern blotting, and then exposed to a radioactively labeled probe parallel to the sequence of interest

  • nitrocellulose paper, a process called southern blotting, and then exposed to a radioactively labeled probe complementary to the sequence of interest

  • cellulose paper, a process called southern blotting, and then exposed to a radioactively labeled probe parallel to the sequence of interest

Question 8 of 25

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Autoradiography does not identify the fragment binding to the probe

Select one of the following:

  • True
  • False

Question 9 of 25

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The polymerase chain reaction (PCR)

Select one of the following:

  • Allows the amplification of any DNA sequence provided some sequence information about the target DNA is known

  • Allows the amplification of any DNA sequence without the sequence information about the target DNA

  • Separates DNA fragments

  • Consist of target DNA and does not require heat in order to stabilize the DNA polymerase

Question 10 of 25

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PCR consist of three steps that are repeated until the desired degree of amplification is achieved. What is the first step?

Select one of the following:

  • The mixture is cooled to a temperature determined by the experimenter (55 degrees Celsius), to allow the primers to anneal to the DNA

  • The duplex containing the target sequence and the flanking regions (primers) is cooled to 80 degrees to generate single strands

  • The duplex containing the target sequence and the flanking regions (primers) is heated to 80 degrees Celsius to generate single strands

  • The mixture is heated to 72 degrees Celsius, allowing the polymerase to synthesize DNA

Question 11 of 25

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PCR is a powerful technique in medical diagnosis, forensics, and studies of molecular evolution

Select one of the following:

  • True
  • False

Question 12 of 25

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Instances of genetic variation

Select one of the following:

  • Polymorphisms, does not correlate with the emergence of a disease

  • Polymorphisms, sometimes correlate with the emergence of a disease

  • Polymorphisms, sometimes correlate with the disappearance of a disease

  • Polymorphisms, does not correlate with the disappearance of a disease

Question 13 of 25

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A vector,

Select one of the following:

  • a piece of RNA readily taken up and replicate by bacteria, is cleaved by the same restriction enzyme used to generate the fragment

  • a piece of DNA readily taken up and replicate by bacteria, is cleaved by the same restriction enzyme used to generate the fragment

  • a piece of RNA ligase readily taken up and replicate by bacteria, is cleaved by the same restriction enzyme used to generate the fragment

  • a piece of DNA ligase readily taken up and replicate by bacteria, is cleaved by the same restriction enzyme used to generate the fragment

Question 14 of 25

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DNA ligase is used to separate the fragment and the vector, generating recombinant DNA

Select one of the following:

  • True
  • False

Question 15 of 25

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Common Vectors are

Select one or more of the following:

  • DNA ligase

  • bacterial plasmids

  • bacteriophage lambda

  • primers

Question 16 of 25

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If the DNA fragment of interest does not contain appropriate restriction sites for vector insertion, a chemically synthesized linker DNA with the required restriction site is added to the fragment

Select one of the following:

  • True
  • False

Question 17 of 25

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Plasmids and lamda phage are choice vectors for DNA cloning in bacteria

Select one or more of the following:

  • Specifically designed plasmids called cloning vectors allow for efficient deletion of DNA as well as replication

  • Specifically designed plasmids called cloning vectors allow for efficient insertion of DNA as well as replication

  • cloning vectors may not have a polylinker region

  • cloning vectors may have a polylinker regions

  • cloning Vectors contain promoters that facilitate transcription of the insert and sequences that allow translation

  • cloning Vectors contain promoters that facilitate transcription of the deletion and sequences that allow translation

Question 18 of 25

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Reporter genes, such as antibiotic-resistance genes, in the vector make identification of vectors with the inserted DNA harder

Select one of the following:

  • True
  • False

Question 19 of 25

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Lamda phage (viruses that infect bacteria) are

Select one or more of the following:

  • cloning vectors

  • can infect a cell and cause lysis or become stably integrated into the host DNA (lysogenic pathway) and be replicated indefinitely

  • Environmental changes does not cause the switch from lysogenic to lytic pathway

  • complementary DNA prepared from mRNA

Question 20 of 25

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Complementary DNA can also be inserted into expression vectors

Select one of the following:

  • True
  • False

Question 21 of 25

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Expression vectors are

Select one of the following:

  • plasmids or λ phage that have powerful promoters for transcription as well as a segment of DNA that encodes a ribosome-binding site that is expressed with the mRNA encoded by the cDNA.

  • plasmids or λ phage that have powerful promoters for translation as well as a segment of DNA that encodes a ribosome-binding site that is expressed with the rRNA encoded by the cDNA.

  • plasmids or λ phage that have powerful promoters for transcription as well as a segment of DNA that encodes a ribosome-binding site that is expressed with the tRNA encoded by the cDNA.

  • plasmids or λ phage that have powerful promoters for translation as well as a segment of DNA that encodes a ribosome-binding site that is expressed with the tRNA encoded by the cDNA.

Question 22 of 25

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Expression vectors allow for not only transcription but also translation of the cDNA library

Select one of the following:

  • True
  • False

Question 23 of 25

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Replica plates are made of a λ phage containing an expression vector, and the plaques are probed with an antibody for the protein of interest.

Select one of the following:

  • True
  • False

Question 24 of 25

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Human gene therapy holds great promise for medicine

Select one of the following:

  • Gene therapy, the deletion of functional genes into cells to replace mutated genes

  • Gene therapy, the insertion of functional genes into cells to replace mutated genes

Question 25 of 25

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Severe combined immunodeficiency (SCID)

Select one or more of the following:

  • Is an example of gene therapy

  • is an example of a phage

  • has been treated by infecting cells with a functional version of the mutated enzyme, adenosine deaminase

  • has been treated by curing cells with a functional version of the mutated enzyme, adenosine deaminase

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Chapter 5 Exploration of Genes Relies on Key tools

Amy Arce
Quiz by , created about 1 year ago

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Amy Arce
Created by Amy Arce about 1 year ago
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