What is the main difference between apoptosis and necrosis?
In necrosis release of cell contents is controlled
In apoptosis it's not
In apoptosis release of cell contents is controlled
In necrosis it's not
What protein condenses during apoptosis
What form are caspases usually found in?
Inactive, with their DED domain tails intact
Active, with their DED domain tails intact
Inactive, with their DED domain tails removed
Active, with their DED domain tails removed
What is required for caspase activation?
Addition of a DED domain tail
Removal of the DED domain tail
What are the roles of the 2 main caspases?
3 is the initation
8 does most damage
8 is the initiator
3 does most damage
Which of the following are survival/protector proteins that inhibit caspase activation?
BAX, BAD, PUMA
Bci-2, Mcl1, A1
What is required for ligand acitvated apoptosis?
For the receptors to contain DED domains
For the receptors to be bound to caspases
For the receptors to be expressed only in certain areas of the cell membrane
What protein inhibits the activation of apoptic receptors?
Name an apoptic receptor
How does the receptor activate caspase 8?
By recruiting it and cleaving off the DED domain
By recruiting it and adding a DED domain
Name the two methods caspase 8 uses to destroy the cell
Activation of Caspase 3
Deactivation of Caspase 3
Damage to mitochondria, which releases Cyt. C
What does mitochondrial damage do to Casp 9?
What happens when essential growth factors are withdrawn from the cell?
PI3K cannot activate
PKB cannot activate
Casp 8 cannot activate
How does PKB inhibit apoptosis?
Releases Bad from Blc-2
Releases Blc2 from Bad
Identify methods which cells can use to evade apoptosis
Decreased Fas receptor expression
Inc Blc2 & decrease bax/bad expression
Loss of Casp 8
Increased Flp expression
Soluble fas to causes apoptosis in t-cells
Large amounts of Fas ligand produced to kill t-cells