Tenet 1- The membrane should be permeable to protons
Tenet 2 - An electron transport chain powers the pumping of protons across a cell membrane
Tenet 3- Energy transducing membranes must have special systems allowing ions and metabolites to pass (to maintain osmotic pressure in the case of a high membrane potential)
Tenet 4- The ATP synthase should NOT also act as an ATP translocator
Why does disrupting the inner mitochondrial membrane increase alkylisation of the medium?
The inner membrane becomes more permeable and protons enter the mitochondria down the concentration gradient
The inner membrane becomes more permeable and protons leave the mitochondria down the concentration gradient
Why does O2 injection acidify the membrane and prove chemiosmotic theory?
The O2 allows protons to be pumped out of the inner membrane into the solution, acidifying it
The O2 allows protons to be pumped out of the solution into the mitochondria acidifying it
The medium then alkylises as they flow back in
The number of protons pumped is substrate dependent
What is this the symbol for in the protonmotive force equation?
Change in electrical potential
Change in pH
The Farraday constant
Put the Protonmotive force equation in order; Change in PMF = _ - (_ x _)
A = Farraday constant
B = Change in pH
C= Change in electrical potential
Short circuiting the protonmotive force generation system causes the energy to be released as heat
The number of protons pumped in a full cycle is cell dependent