What is the difference between Stage 3 & Stage 4?
Stage 3 is between ADP addition and ADP running out
Stage 3 is between ADP running out and oxygen running out
Stage 4 is between ADP running out and oxygen running out
Stage 4 is between O2 running out and ADP running out
Measuring oxygen consumption is done by looking at the change in the X co-ordinates
Once you've worked out the change in O2 concentraction, how do you calculate nmol O2 /min/mg protein
Divide by mg of protein
Divide by 60
Divide by O2 consumed
Assume a change in the axis of 15mm, and a protein concentration of 1.4mg. Calculate the Rate
25.7 nm Oxygen/min/mg protein
10.7 nm Oxygen/min/mg protein
50.4 nm Oxygen/min/mg protein
What is the equation for the respiratory control ratio?
Rate of phase 4/ Rate of phase 3
Rate of phase 3/ Rate of phase 4
Rate of phase 3 x Rate of phase 4
ADP/O ratio = Moles of ADP added/Moles of ADP consumed
To convert from O2 to O, what must you do?
What must be checked before calculating the ADP/O ratio?
That both sides are in the same molar concentration
That the oxygen consumed has been converted into nm
That the ratio is positive