In Flow Cytometry, cell size is measured as they pass through laser beams
Why is this area left?
Excludes cells with low CD45
Excludes small cells
Excludes cells too large to pass through
In Flow Cytometry, the Ig tested for varies depending on which cell you're measuring
For which cell would you use an alkaline reagent?
Normal range of W.B.C's is 4-11 (x10^7/l)
What is to be expected in a Settling test from a leukaemia patient?
Thinker plasma layer
Thicker buffy coat layer
Thicker erythrocyte layer
Why is a strong lysing solution added to a W.B.C count?
Like R.B.C's, W.B.C's are counted using current impedence
What are the two populations (peaks) shown here?
What two things are mapped in D.I.F.F?
What cells are being pointed at on the graph?
Cellpack & Stromatolyers 3D do what?
Speed R.B.C's lysis
List some causes of Neutropenia
Overwhelming bacterial/viral/funal/parasitic infections
Chronic infection/inflammation and increased cytokinase can lead to high...
Basophilia is caused by
Eosinophilia is caused by allergic infections, parasitic infections and drug hypersensitivity
Name some causes of Lymhpocytopenia
Name the three tests done for any Leucocytic disorder
Full blood count & DFF
Paul Bunnell Test
Why does Clonality indicate a severe pathology?
A single cell type is proliferating massively
Shows a lack of diversity across leucocytes
Shows massive infection
What markers do you test for when looking for Clonality?
Alpha, beta & gamma
In AIDS, you perform flow cytometry for Kappa/Lambda markers
The Paul Bunnel test is done for...
Name the cause of Haematological Malignancies