Why was it important to keep replacing the agar in the water bath when making the slides?
What was done to make sure that the agar stuck to the slides even when the slides were upside down in the electrophoresis tank?
Other than not separating proteins accurately, what effect could partially solidified agar have on the results?
How was the bromophenol blue dye added to the agar slide?
What is the function of the bromophenol blue dye? How did it do this?
Which electrode in the electrophoresis tank did the serum proteins and bromophenol blue dye travel towards? Why?
How was the number of milli-amps (current) applied to the slides calculated?
With regard to Ag-Ab interactions, what was first spread throughout the agar by electrophoresis?
With regard to Ab-Ag interactions, what creates the strongest bond between the two?
Given that the charge of the proteins determines the direction of travel, what determines the distance travelled?
At what point in the assay did the slides cease to be identical?
Given that certain antibodies are used to look for certain antigens, what quality can the technique be said to have?
Why is a dye not necessary to visualise the Ab-Ag interactions?
What is a precipitin?
Why does visible precipitation of antibodies and antigens only occur in the region of equivalence?
What is the purpose of immunoelectrophoresis? Therefore, which word can be used to describe the technique?
In what way is immunoelectrophoresis a sort-of quantitative technique?
Why is IEP a limited technique?
How might IEP be useful in monitoring therapy?
What is hypergammaglobulinaemia?
List some chronic infections that hypergammaglobulinaema can be indicative of.
What type of hypergammaglobulinaemia would be present in multiple myeloma compared with the other conditions listed?
Why does albumin travel the furthest through the agar gel?
What does the arc of the precipitin curve on the agar slide represent?
In order for cross-linked antibody-antigen complexes to be formed, what must be true of the antibodies?
What equipment is required to produce the trough and wells on the agar slides?
