The Quantitative Precipitin Curve

What were the pellet and supernatant used for in this/these assay(s)?

Why does excess antigen not result in maximal precipitation of antibody and antigen?

What is the quantitative precipitin curve used to determine?

What two things need to be known in order to calculate the concentration of antigen/antibody in a quantitative precipitin curve?

What piece of equipment was used to establish the level of precipitation at the different antigen concentrations?

What would you expect the Ouchterlony slides for BSA and anti-BSA to show either side of the equivalence point?

What type of reaction/technique is this?