Why are enzymes immobilised during bioprocessing?
They are insoluble and easier to reuse and recover
To prevent wasting the enzymes
To decrease the yield of products
They are soluble and harder to reuse
How are enzymes immobilised?
bonded to each other
bonded or attached to an insoluble support
fixed to a substrate
enclosed in a gel or membrane
Trapping the enzymes/cells in a gel is the most common technique used for immobilising enzymes
In the production of alcohol, what gel are the enzymes/cells fixed to?
What solution are the beads of the sodium alginate-cell/enzyme mixture soaked in during alcohol fermentation?
What role does calcium chloride play in alcohol fermentation?
It allows sucrose to enter the alginate pores.
It causes the alginate to enclose the immobilised yeast cells.
It prevents the yeast to pass through the pores.
Choose the advantages of immobilised cells used in bioprocessing?
It does not damage the cells or affect their ability to carry out the reaction.
It is a costly process.
The immobilised cells can easily be recovered from the culture medium at the end of the process.
Immobilised cells substantially increase the need for filtration.
Immobilised cells are being increasingly used over immobilised enzymes.