Re: the Cell Counting lab, you should be able to: • given dimensions of a field(s) (that is, the “squares”) to be counted, calculate the volume in mm3 and convert to mL

First volume of square is DxWxH
then 1 cubic meter = 0.001ml

• take an accurate cell count from a haemocytometer

• from the count, determine cells/mL based on the volume of the field (i.e. the haemocytometer squares) considered in the count

1cm3 = 0.001ml pretty much 10^4

Average viable cell count per square x Dilution Factor x 104/ • from a determination of cells/mL in the field, correct if necessary for any initia dilution of the suspension made prior to loading the haemocytometer
times back by diloution factor
• given a cell/mL count in a “concentrated” suspension, determine the dilution necessary to plate out cells at a more “dilute” density in a given volume of culture medium?? • determine the volume of the drug to apply and calculate from its stock concentration the concentration in the cell culture Remember that apart from the volume calculation, everything else you have done already, although in different contexts (i.e. determining amounts and concentrations; making dilutions; etc.).

	Created by celiacrook93 about 10 years ago

Note on PSB ELISA/Hemo , created by celiacrook93 on 24/02/2014.

PSB ELISA/Hemo