Of the following polymorphisms which are single nucleotide differences that are seen between two genomes?
Restriction fragment length polymorphisms
Short tandem repeats
Variable number tandem repeats
Single nucleotide polymorphisms
Which of the following polymorphisms can be detected through Southern blot?
Long interspersed nucleotide sequences
What does it mean, that a locus is homozygous?
many different alleles
two alleles that are the same
two alleles that are different
one allele have been deleted
The following is a description of which method.
Patients DNA was isolated and cut with HaeIII. Product of the restriction digest was resolved by gel electrophoresis and transferred to a membrane. Then the membrane was exposed to hybridization probes, and the resulting band pattern was compared with that seen in another sample of DNA.
RFLP by Southern blotting
VNTR by PCR
SNP by sequencing
STR by PCR
SNP by Western Blot
FGA, D8S1179, and D2S11 are examples of which of the following?
Which of the following is used as a marker of gender?
How many loci are considered core loci by the Federal Bureau of Investigation in the Combined DNA Indexing System?
When two sources of DNA are compared by STR analysis in a crime scene, how many differences between alleles will determine that the two sources are different?
Which of the following polymorphisms can be used to compare DNA samples derived from males but not females?
Which of the following STRs is a Y-STR?
A child has disputed paternity. The child has the following STR haplotype:
TPOX = 7/8; D5S818 = 11/12; D13S317 = 9/10; vWA = 11/12; D21S11 = 27/28.
Alleged Father 1 has the following STR haplotype: TPOX = 6/10; D5S818 = 9/13; D13S317 = 9/14; vWA = 14/16; D21S11 = 30/33.
What can be said of Alleged Father 1?
He is the father of the child
He cannot be excluded as the father
He is excluded as the father.
More STRs need to be analyzed before a conclusion can be made.
An individual who has two populations of cells that were derived from two different zygotes is called a:
A woman has been raped by an unknown man. Which of the following laboratory procedures can be used to more accurately analyze residual DNA left by the man in an effort to identify him?
DNA found at the scene of the crime was determined to have one peak at 212 bp at the amelogenin locus. The source of this DNA is which of the following?
Impossible to tell from one locus
This locus does not discriminate between males and females
STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons?
To determine whether the donor and recipient are a match
To determine the likelihood of graft-versus-host disease
To find informative alleles for post-transplantation studies
To add their STR haplotypes to a national database
STR analysis was performed on a bone marrow donor and recipient pair with the following results. Which loci are informative?
vWA and TH01
TH01 and D13S317
D16S539 and F13A01
vWA and D13S317
Bone marrow has been transplanted into a recipient. STR analysis was performed on whole blood from the recipient 6 months after the transplant by PCR, followed by capillary gel electrophoresis with fluorescence measurement of amplicons. For the TPOX locus, the donor and recipient do not share alleles. The fluorescence of the TPOX locus for the donor peak was 15693, and the fluorescence of the TPOX locus for the recipient peak was 5329. What is the percentage of recipient cells remaining?
Two people have what percentage of DNA sequence that differs between them?
Two middle-age women who had been adopted into different families as infants discover circumstantially that they may have the same biological mother. Which of the following analyses would give the best information toward proving their maternal lineage?
Consider the following STR analysis:
Which of the child alleles are inherited form the father?
D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 9
D3S1358: 4, vWA: 5, FGA: 20, TH01: 11 , TPOX: 10, CSF1PO: 13, D5S818: 3, D13S317: 6
D3S1358: 15, vWA: 17, FGA: 23, TH01: 6 , TPOX: 11, CSF1PO: 12, D5S818: 10, D13S317: 10
D3S1358: 10, vWA: 17, FGA: 24, TH01: 6 , TPOX: 10, CSF1PO: 12, D5S818: 10, D13S317: 9
D3S1358: 15, vWA: 18, FGA: 24, TH01: 10 , TPOX: 11, CSF1PO: 12, D5S818: 12, D13S317: 10
Basked on the results which of the alleged fathers (AF) is the father?
A child and an alleged father (AF) share alleles with the following paternity index:
What is the combined paternity index from these three loci?
With 50% prior odds, what is the probability of paternity from these three loci?
What is a traditional definition of a polymorphism?
The genets variation must be present in at least 1 % of the population.
The genets variation must be present in at least 5 % of the population.
The genets variation that no longer is present in the population.
The genets variation can not be present in majority of the population.
Which of the following genomic variations is the hardest to detect?
When comparing a generic similarity go two random individuals, what percentage of their genome is identical?
RFLP stands for?
Restriction fragment length polymorphism
Rapidly Fusing length polymorphisms
Rescue factor logical polymorphisms
Redundant fragment length polymorphism
Closely linked markers are used for:
Accurate mapping of regions associated with phenotypic characteristics
Finding the length of a sequence
Location of the centromere
Defining the promoter
Disabling the binding of transcription factors
What is a definition of a haplotype?
A block of closely linked SPS on the same chromosome that tend to be inherited together.
A half of a chromosome.
Chromosome with only one functioning allele.
Allele that gives a disfiguring phenotype.
Mitochondrial DNA is____
circular double stranded
circular single stranded
linear double stranded
linear single stranded
Genes encoded on the mtDNA include:
22 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
30 tRNA genes, 2 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 6 ribosomal RNA genes, and 12 genes coding for components of the oxidation-phosphorylation system.
22 tRNA genes, 2 ribosomal RNA genes, and 60 genes coding for components of the oxidation-phosphorylation system.
Mitochondrial DNA follow which type of inheritance?