What is the Michaelis-Menten model for enzyme kinetics?
What is Kcat a measure of?
What are the units of Kcat?
What is the equation for Kcat?
What is Vmax?
Draw a Michaelis-Menten plot of v versus [S].
What is the Michaelis Menten equation? What do all the parts of it stand for?
Which has a higher affinity for substrate binding? High or low Km?
What does Km equal on the michaelis-menten graph?
What are the units for Km?
Draw a Lineweaver-Burk Double Reciprocal plot.
What is a competitive inhibitor? How can it be overcome?
What is a non-competitive inhibitor? How can it be overcome?
How does the Lineweaver burk plot change with competitive and non-competitive inhibitors?
What is Ki?
What factor is the slope of a lineweaver-burk plot increased by in presence of competitive inhibitor?
Does low Ki indicate strong or weak enzyme binding?
What happens to Vmax and Km in the presence of a non-competitive inhibitor?