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Taken from Ralston, A. and J. Rossant (2010). "The genetics of induced pluripotency." Reproduction 139(1): 35-44.
Plate cells at density of 500,000 onto 10cm dishes - 6 well plate = 9.61cm2
Day 1
Days 2-4
Day 5
Day 6
Day 7-11
Day 12-16
Day 17
Days 18+
Scaling up of selected clones
Pick clones/colonies
Grow on FIAU
Allow for turn-over of vector
Electroporation
New Page
Piggy Bac excision
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