Chemiluminescence

Assay

Analyte

Assay Buffer

Multiplex

Pos Control

Neg Control

Signal to Background Ratio

Assay Format

% Inhibition

Assay Design

Assay Development

Assay Validation

Accuracy

Precision

LOD

LOQ

Specificity

Linearity

Range

Ruggedness

Robustness

Direct Assay

Competitive Assay

Absorption

Absorbance

Transmittance

Fluorescence Quenching

Self quenching

Quencher

Quantum Yield

Choices for detection methods

Optimization Parameters for ELISA

Assessing an assay

Parameters essential for immunoassay validation

Advantages/Disadvantages of ELISA

Epitope

Two types of Epitopes

Monoclonal Antibodies

Polyclonal Antibodies

Details about Polyclonal

Details about Monoclonal

Different ELISA formats

Direct vs Indirect ELISA

Parameters to optimize a sandwich ELISA

Methods of coating wells with capture antibody or antigen

Protein Tags

Steps for indirect sandwich ELISA

Choices of detection method for ELISA

2 Most common types of enzymes used for ELISA

Mechanism of fluorescence

Photobleaching

Fluorescence detection

Monochromator - fluorescence

Filter-based - flourscence

environmental effects on fluorescence

Background signals that limit fluorescence sensitivity

Importance of reading Absorbance assay at max wavelength

Spectrophotometric assay

Flourescence

Coupled enzyme assays

Malachite green detection of phosphate