Anneal Primers

Nestled primers

Melt DNA at...

Primers bind after...

Anneal at...

Extend primers at...

The stages are repeated...

The ends of the amplified DNA represent

DNA Polymerases Vary In

Uses of PCR- Viruses

Uses- Cloning

Uses- DNA

Quirk of Taq Polymerase

T- Vectors

TOPO Isomerase

Introduction of a Restricton Site

Reverse Transcriptase PCR

Reverse Transcriptase PCR- Primers

Reverse Transcriptase PCR- Tth Thermostable DNA Polymerase

Quantitative PCR

DNA Production in PCR

Light Cycler Assay Probes

Light Cycler Assay- Fluorescence

Use of Taqman Assay

Use of Taqman Assay Probes

Use of Taqman Assay Amplification

Molecular Beacons

Binding of Molecular Probes