RF1- CaCl2, MgCl2, KCl

RF2- Mops, KCl, CaCl2, glycerol

LB

Competent E. coli

Tryptone (LB)

Yeast extract (LB)

NaCl (LB)

Agar (LB)

Ampicillin

IPTG

X-Gal

Transformation efficiency

Cloning vectors

pBluescript

Plasmid extraction by alkaline extraction method

Restriction enzymes used to cut plasmid

DH5-

Electrophoresis buffer

Alkaline Phosphatase (AP)

Ethidium Bromide

Loading Dye and glycerol

DNA (plasmid) purification

PCR- Materials

PCR- process

Insert

Restriction enzymes used to cut insert and pair to sticky ends of plasmid

Primer design

Primer design cont.

P

nanodrop

DNA ligase

Ligase mechanism

Optimal conditions for succesfull ligation

Potential problems with ligation

Antibody נוגדן

Conditions of Elisa

Pros of Elisa method

Uses for Elisa method

Non- competitive Antibody test
(sandwich)

Non- competitive antigen test
(sandwich)

Competitive test for antibody

Competitive test for antigen