Microbiology Practical F

Organisms that can only grow inside another living host.

Viruses that infect bacteria.

Viral replication cycle that ends in the death of the host cell.

Viruses that infect bacteria.

Organisms that can only grow inside another living host cell.

Viral replication cycle that ends in death of its host cell.

Viral replication cycle that ends in death of its host cell.

Organisms that can only grow inside another living host cell.

Viruses that infect bacteria.

Use ordinary media to grow bacteria but, a bacteriophage needs a host cell.

Use ordinary media to grow bacteriophages but, bacteria needs a host cell.

To obtain a countable plate of 30 - 300 plaques.

To obtain a countable plate of 30 - 300 colonies.

To obtain a countable plate of 30 - 300 individual viruses.

An area of clearing on an agar representing a spot where a virus landed, infected a bacteria and lysed it.

An area on an agar representing a spot where a bacteria landed, infected a virus and lysed it.

An area on an agar representing a spot where a virus landed, infect a yeast and lysed it.

It is the host cell for the T4 bacteriophage

It is the host cell of the T4 bacteria.

It is the host cell of the T4 fungus.

It can grow as a yeast and as a mold.

It can grow as a yeast and a virus.

It can grow as a mold and a virus.

Fungi grow faster, at a lower temperature and at a lower pH than bacteria.

Fungi grow faster, at a higher temperature and at a higher pH than bacteria.

Fungi grow slower, at a lower temperature and at a lower pH than bacteria.

Fungi grow slower, at a higher temperature and at a higher pH than bacteria.

Gram (+) cocci in clusters

Gram (-) cocci in clusters

Staphylococcus is gram (+) in clusters and Micrococcus is gram (-) in pairs

Staphylococcus is gram (-) in clusters and Micrococcus is gram (+) in pairs

Skin and mucus membranes

Intestines

Gastrointestinal tract

Skin and hair

Skin and mucus membranes

Intestines

Gastrointestinal tract

Skin and hair

Carrier

MRSA

Nosocomial

Giver

MRSA

Carrier

Nosocomial

Multiple Resistant Streptococcus Aureus

Nosocomial

Carrier

Giver

Catalase

They are opportunistic pathogens.

All the time

Never

Staph are larger, convex, opague and pigmented, Strep are smaller, translucent to slightly opaque.

Strep are larger, convex, opague and pigmented, Straph are smaller, translucent to slightly opaque.

Staph are smaller, convex, opague and pigmented, Strep are larger, translucent to slightly opaque.

Catalase

Streptocase

Staphoolase

C polysaccharide extracted from the cell walls

Peptidoglycan extracted from the cell walls

Lipids extracted from the cell walls

Streptococcus pyogenes

Streptococcus aureus

Streptococcus epidermidis

Streptococcus agalaticae

Streptococcus agalaticae

Streptococcus aureus

Streptococcus pyogenes

Streptococcus cocci

Gastrointestinal tract

Skin and mucus

Liver

Kidneys

Vancomycin resistant enterococcus

Vancomycin resistant ebolacoccus

Vancomycin resistant enteroboccus

Alpha hemolytic streptococci other than streptococcus pneumonea

Beta hemolytic streptococci other than streptococcus pneumonea

Alpha heolytic staphylococci other than staphylococcus pneumonea

Beta heolytic staphylococci other than staphylococcus pneumonea

Strep throat

Community-acquired pneumonia

Neonatal septicemia

Urinary tract infection

Neonatal septicemia

Strep throat

Urinary tract infection

Community-acquired pneumonia

Urinary tract infection

Dental cavities

Strep throat

Neonatal speticemia

Community-acquired pneumonia

Neonatal septicemia

Urinary tract infection

Strep throat

Dental cavities

Strep throat

Urinary tract infection

Neonatal septicemia

It can grow as a yeast and as a mold

It can grow in pairs.

It can grow as coccus or bacillus.

Fungi grow slower, at a lower temp and lower pH than bacteria

Fungi grow faster, at a higher temp and higher pH than bacteria

Fungi grow slower, at a higher temp and lower pH than bacteria

Fungi grow faster, at a lower temp and higher pH than bacteria

Vegetative hyphae grow on or down into the agar surface; aerial hyphae grow above the agar surface.

Aerial hyphae grow on or down into the agar surface; vegetative hyphae grow above the agar surface.

Sporangipospores are inside of a sac and condidiospores are the end of hyphae.

Condidiospores are inside of a sac and sporangipospores are the end of hyphae.

Glucose

Lactose

It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is differential because it contains lactose making it differential for lactose fermentation.

It is selective because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is differential because it contains lactose making it differential for lactose fermentation.

It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (+) organisms. It is selective because it contains lactose making it differential for lactose fermentation.

It is differential because it contains bile salts and crystal violet which inhibit the growth of gram (-) organisms. It is selective because it contains lactose making it differential for lactose fermentation.

1

2

4

Gram (-)

Gram (+)

Lactose

Bile salts and crystal violet

Blood

Gram (+)

Gram (-)

Bile salts and crystal violet

Blood

Lactose

Streptococcus are microaerophiles

Streptococcus are anaerobes

To make sure we get both organisms from the mixed culture

To make sure enough sample is collected

To make it easier to inoculate the media

It's a neutral media that all bacteria like

It is a selective media that only gram (-) bacteria like.

It is a selective media that only gram (+) bacteria like.

You have to develop a dichotomous key for each of your unknown organisms and we have two organisms.

You have to develop a dichotomous key for each depending on the outcome of growth on the selective media.

Part of the normal flora of the intestinal tract

Bacteria that live inside another organism.

Substrates are incorporated into the culture media that the enzymes act upon and that along with indicator system lets you see the products.

Enzymes are incorporated into the media and an indicator systems lets you see if they match the organism.

Collects gas

Releases gas

Keeps tube from exploding

So you can see the hemolysis better

To see if the bacteria like to grow in or on the agar

In a candle jar because it's a microaerophile

In the air because it's an aerobe

The alpha Streptococcus are bile esculin (-) and the Enterococcus faecalis is bile esculin (+)

The alpha Streptococcus are bile esculin (+) and the Enterococcus faecalis is bile esculin (-)

Staphylococcus is catalase (+) and Streptococcus is catalase (-)

Staphylococcus is catalase (-) and Streptococcus is catalase (+)

# of plaques x dilution x 10^-1 = _________ PFU/mL

# of plaques x dilution x 10^1= __________ PFU/mL

Staphylococcus aureus

Staphylococcus epiderminis

Micrococcus roseus

Micrococcus luteus

Staphylococcus epidermidis

Stapylococcus aureus

Micrococcus roseus

Micrococcus luteus

Micrococcus roseus

Micrococcus luteus

Staphylococcus aureus

Staphylococcus epidermidis

Micrococcus luteus

Micrococcus roseus

Staphylococcus aureus

Staphylococcus epidermidis

It is positive for bile esculin

It is negative for bile esculin

Streptococcus mitis

Streptococcus pneumoniae

Streptococcus pyogenes

Group C streptococcus

Streptococcus pneumoniae

Streptococcus mitis

Streptococcus pyogenes

Group C Streptococcus

Group C Streptococcus

Streptococcus pyogenes

Streptococcus pneumoniae

Streptococcus mitis

Streptococcus pyogenes

Streptococcus mitis

Streptococcus pneumoniae

Group C Streptococcus