Questão 1
Questão
In microbiology the term growth usually refers to an increase in
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a microbe's size.
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the number of microbial cells.
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the amount of ATP produced.
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the number and size of microbial cells.
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the number and size of microbial cells and the amount of ATP produced.
Questão 2
Questão
A cell that uses an organic carbon source and obtains energy from light would be called a
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photoheterotroph
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lithoautotroph
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photoautotroph
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chemoheterotroph.
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chemoautotroph
Questão 3
Questão
A(n) ______________ organism is one that requires oxygen for growth.
Questão 4
Questão
An aquatic microbe that can grow only at the surface of the water is probably which of the following?
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a phototroph
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a heterotroph
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a chemotroph
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a lithotroph
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an anaerobe
Questão 5
Questão
Which of the following forms of oxygen is detoxified by the enzyme catalase?
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singlet oxygen
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hydroxyl radical
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peroxide anion
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superoxide radical
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molecular oxygen
Questão 6
Questão
All of the following are used to protect organisms from the toxic by-products of oxygen EXCEPT
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carotenoids.
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superoxide dismutase
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peroxidase
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protease.
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catalase.
Questão 7
Questão
A microbe that grows only at the bottom of a tube of thioglycollate is probably a(n)
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obligate aerobe.
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facultative anaerobe.
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aerotolerant anaerobe.
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microaerophile.
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obligate anaerobe.
Questão 8
Questão
Nitrogen is growth limiting nutrient for many organisms because
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it is necessary for the biosynthesis of amino acids
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it is required for synthesis of nucleotides.
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it is required for lipid synthesis.
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only a small number of bacteria are able to extract it from the atmosphere.
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only a few microbes can extract it from the atmosphere, but all organisms require it for amino acid and nucleotide synthesis.
Questão 9
Questão
Which of the following growth factors would NOT be required by microbes which do not utilize electron transport chains?
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heme.
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selenium.
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NADH.
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vitamins.
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amino acids.
Questão 10
Questão
At temperatures higher than the maximum growth temperature for an organism,
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proteins are permanently denatured.
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membranes become too fluid for proper function.
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hydrogen bonds within molecules are broken.
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hydrogen bonds are broken, and proteins are proteins are permanently denatured.
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hydrogen bonds are broken, proteins are denatured, and membranes becomes too fluid.
Questão 11
Questão
Human pathogens are classified as
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thermoduric.
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thermophiles.
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mesophiles.
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hyperthermophiles.
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psychrophiles.
Questão 12
Questão
The term barophile refers to which of the following growth requirements?
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hydrostatic pressure
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temperature
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nitrogen source
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pH
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osmotic pressure
Questão 13
Questão
Which of the following organisms would be most likely to contaminate a jar of pickles?
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a neutrophile
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a thermophile
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an acidophile
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an obligate anaerobe
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a mesophile
Questão 14
Questão
Organisms that can grow with or without oxygen present are
Questão 15
Questão
A fastidious organism might be grown on which of the following types of media?
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transport media
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reducing media
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enriched media
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differential media
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selective media
Questão 16
Questão
Obligate anaerobes may be cultured in the laboratory
Questão 17
Questão
Joan wants to discover a microbe capable of degrading an environmental contaminant. Which of the following refers to the process she should use?
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chemostat
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quorum sensing
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enrichment culture
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nitrogen fixation
Questão 18
Questão
A microbiologist inoculates a growth medium with 100 bacterial cells/ml. If the generation time of the species is 1 hour, and there is no lag phase, how long will it be before the culture contains more than 10,000 cells/ml?
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24 hours
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7 hours
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2 hours
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3 hours
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10 hours
Questão 19
Questão
An epidemiologist is investigating a new disease and observes what appear to be bacteria inside tissue cells in clinical samples from victims. The scientist wants to try to isolate the bacteria in the lab. What culture conditions are most likely to be successful?
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culturing on blood agar plates
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inoculation of EMB plates
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incubation in a candle jar
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inoculation of cell cultures
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inoculation of a minimal medium broth
Questão 20
Questão
Which of the following measurement techniques would be useful to quantify a species of bacteria that is difficult to culture?
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membrane filtration
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viable plate counts
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MPN
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microscopic counts
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metabolic activity
Questão 21
Questão
MacConkey agar plates represent __________ medium.
Questão 22
Questão
A Petroff-Hauser counting chamber is
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a glass slide containing an etched grid for counting microbes directly using a microscope.
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a device that counts cells as they interrupt an electrical current.
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a device that measures the amount of light that passes through a culture.
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an apparatus that traps bacterial cells on a membrane filter where they can be counted.
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a device used to count numbers of bacterial colonies on a Petri plate.
Questão 23
Questão
Another term for the logarithmic growth of bacterial cells is
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generation time.
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exponential growth.
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arithmetic growth.
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absorbance.
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binary fission.
Questão 24
Questão
A device that removes wastes and adds fresh medium to bacterial cultures in order to prolong the log phase of a culture is called a(n)
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Coulter counter.
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cytometer.
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spectrophotometer.
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pellicle.
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chemostat.
Questão 25
Questão
A specimen of urine is determined to contain 30 bacterial cells per microliter. How many cells would be present in a milliliter?
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3
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300
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30,000
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3,000
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30 million
Questão 26
Questão
Which of the following is NOT a direct method for measuring the number of microbes in a sample?
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viable plate counts
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turbidity
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Coulter counter
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MPN
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membrane filtration
Questão 27
Questão
During which growth phase are bacteria more susceptible to antimicrobial drugs?
Questão 28
Questão
The use of salt and sugar in preserving various types of foods is an application of which of the following concepts?
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nitrogen fixation
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osmotic pressure
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pH
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hydrostatic pressure
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quorum sensing
Questão 29
Questão
A clinical sample labeled as "sputum" was collected from
Questão 30
Questão
The method of obtaining isolated cultures that utilizes surface area to dilute specimens is called
Questão 31
Questão
__________ are complex communities of various types of microbes that adhere to surfaces.
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Aggregates
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Colonies
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Isolates
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Biofilms
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Media
Questão 32
Questão
A colony-forming unit is the number of cells
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in a colony.
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that produces one colony.
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in a particular specimen.
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that is in exponential phase in a culture.
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that can be placed on a Petri plate.
Questão 33
Questão
All of the following ingredients might be found in complex media EXCEPT
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hydrogen peroxide.
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yeast extract.
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blood
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soy extract.
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milk proteins.
Questão 34
Questão
Sodium thioglycollate is associated with which of the following types of media?
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transport media
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differential media
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complex media
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reducing media
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selective media
Questão 35
Questão
Which of the following quantification techniques can distinguish living cells from dead cells in a culture?
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microscopic counts
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dry weight
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metabolic activity
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a Coulter counter
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turbidity
Questão 36
Questão
During the __________ of growth, new cells are being produced at the same rate as other cells are dying.
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lag phase
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log phase
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death phase
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stationary phase
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intermediate phase
Questão 37
Questão
During the __________ of growth, cells are dying faster than new cells are being produced.
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death phase
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lag phase
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log phase
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stationary phase
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longitudinal phase
Questão 38
Questão
When cells are metabolically active but not dividing, they are in the
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stationary phase.
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log phase.
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lag phase.
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death phase.
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exponential phase.
Questão 39
Questão
Metabolic activity is at maximum level in the __________ of growth.
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lag phase
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log phase
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death phase
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stationary phase
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intermediate phase
Questão 40
Questão
Cells are rapidly growing and dividing during the __________ of growth.
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death phase
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lag phase
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log phase
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stationary phase
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indeterminate phase
Questão 41
Questão
An organism that tolerates high __________ is called a halophile.
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hydrostatic pressure
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salt concentrations
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pH
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carbon dioxide levels
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oxygen levels
Questão 42
Questão
A capnophile is a microorganism that thrives in conditions of high __________.
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carbon dioxide levels
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hydrostatic pressure
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oxygen levels
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pH
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salt concentration
Questão 43
Questão
A microorganism found living under conditions of high __________ is a barophile.
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pH values
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oxygen concentrations
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hydrostatic pressure
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carbon dioxide levels
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salt concentrations
Questão 44
Questão
Microaerophiles are microbes that grow best at low
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carbon dioxide levels.
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pH values
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hydrostatic pressure.
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oxygen levels.
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salt concentrations.
Questão 45
Questão
An acidophile thrives under conditions of low
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carbon dioxide levels.
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salt concentrations.
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oxygen levels.
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hydrostatic pressure.
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pH
Questão 46
Questão
All of the following are associated with nucleic acid structure EXCEPT
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ribose
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hydrogen bonds.
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uracil
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ionic bonds.
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phosphate
Questão 47
Questão
Which of the following is found at the 5 end of a DNA strand?
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a phosphate group
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a hydrogen bond
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a hydroxyl group
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histones
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a methyl group
Questão 48
Questão
The bacterial chromosome is
Questão 49
Questão
Which of the following types of plasmids allows a bacterial cell to kill its competitors?
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virulence factors
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fertility factors
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bacteriocin factors
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resistance factors
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cryptic plasmids
Questão 50
Questão
Which of the following is NOT involved the packaging of eukaryotic chromosomes?
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nucleosomes
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histones
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heterochromatin
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euchromatin
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Okazaki fragments
Questão 51
Questão
Which of the following statements is true of bacterial plasmids?
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They are found in the nucleoid.
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They can replicate autonomously
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They carry genes for essential metabolic functions.
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They are small circular DNA molecules.
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They are small circular DNA molecules that can replicate autonomously.
Questão 52
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break hydrogen bonds between complementary nucleotides.
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synthesize short DNA molecules important for the function of DNA polymerase.
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seal gaps between DNA fragments.
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proofread DNA molecules.
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assist in recognition of promoters during transcription.
Questão 53
Questão
Which of the following statements concerning transcription in bacteria is FALSE?
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It occurs in the nucleoid region.
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Sigma factors are parts of RNA polymerase that recognize promoter regions.
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The same RNA polymerase transcribes primer RNA, mRNA, tRNA, and rRNA.
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Termination is either self-induced or due to the presence of Rho protein.
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There are a variety of sigma factors that affect transcription.
Questão 54
Questão
Which of the following is involved in translation?
Questão 55
Questão
Which of the following is a characteristic shared by DNA and RNA polymerases?
Questão 56
Questão
Which of the following is the strongest base pair?
Questão 57
Questão
A codon is a particular combination of three nucleotides. Therefore, there are __________ possible combinations of the nucleotides A, C, G, and T.
Questão 58
Questão
The AUG codon functions in coding for the amino acid methionine and as a
Questão 59
Questão
Which of the following must be removed from a eukaryotic mRNA molecule before it can be translated?
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promoter
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exon
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intron
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anticodon
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codon
Questão 60
Questão
A charged tRNA first enters the ribosomal __________ site and then moves into the __________ site.
Questão 61
Questão
Which of the following statements regarding eukaryotic transcription is FALSE?
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Transcription occurs in the nucleus, mitochondria, and chloroplasts (if present).
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Multiple transcription factors are required.
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There are four different RNA polymerases.
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Multiple elongation factors are required.
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A guanine is added to the 3 end, and a poly-A tail is added to the 5 end of the mRNA transcript.
Questão 62
Questão
Semiconservative DNA replication means that
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each daughter DNA molecule is composed of one original strand and one new strand.
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nucleotides are constantly being recycled as cells make DNA.
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the cell can proofread its newly synthesized DNA only part of the time.
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the sequence of a DNA molecule is preserved as it is being replicated.
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each strand of a double-stranded DNA molecule is replicated differently.
Questão 63
Questão
Which of the following is NOT a characteristic of Okazaki fragments?
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They are checked for accuracy by DNA polymerase III.
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They make up the lagging strand of replicated DNA.
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They begin with an RNA primer.
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They are joined together by DNA ligase.
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They are longer in eukaryotic cells.
Questão 64
Questão
The proofreading function of DNA polymerase III results in an error rate of __________ during DNA replication.
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1 error in 1,000 bases
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1 error in 100,000 bases
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1 error in 1 million bases
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1 error in 10 million bases
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1 error in 10 billion bases
Questão 65
Questão
Which of the following processes is involved in the "central dogma" of genetics?
Questão 66
Questão
Inducible operons
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are active in the presence of a repressor
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are generally anabolic pathways
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are normally active
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usually require an activator to be transcribed.
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usually require a repressor to be transcribed.
Questão 67
Questão
Which of the following are considered to be frameshift mutations?
Questão 68
Questão
If the codon AAA is changed to AAG, it still codes for the amino acid lysine; this is an example of a
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silent mutation
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nonsense mutation
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frameshift mutation
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gross mutation
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missense mutation
Questão 69
Questão
Which of the following causes mutations by creating thymine dimers?
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nucleotide analogs
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nitrous acid
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ultraviolet light
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benzopyrene
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gamma rays
Questão 70
Questão
DNA damage caused by ethidium bromide results in __________ mutations.
Questão 71
Questão
Which of the following is a DNA repair enzyme activated by visible light?
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transposase
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DNA photolyase
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bacteriocin
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DNA ligase
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primase
Questão 72
Questão
The Ames test proves that a chemical is
Questão 73
Questão
The horizontal transfer process known as transduction
Questão 74
Questão
Frederick Griffith discovered
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transformation
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transposons
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the lac operon
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DNA
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conjugation
Questão 75
Questão
In conjugation, F+ cells
Questão 76
Questão
Another term for the palindromic sequence found at the ends of transposons is a(n)
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complex
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bacteriophage
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insertion sequence
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inverted repeat
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transposase
Questão 77
Questão
Which of the following is characteristic of prokaryotic genomes but NOT eukaryotic genomes?
Questão 78
Questão
Genes are active in a region of the eukaryotic chromosome called
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heterochromatin
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the histone
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the nucleoid
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the plasmid
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euchromatin
Questão 79
Questão
The energy required for DNA replication comes from
Questão 80
Questão
Which of the following is NOT involved in the regulation of the lac operon?
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an inducer
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a repressor protein
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an iRNA
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glucose
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cyclic AMP
Questão 81
Questão
Genetic elements known as promoters are initiation points in the process of
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DNA replication
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transcription
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translation
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mutation repair
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transformation
Questão 82
Questão
Codons are recognized during
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translation
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transcription
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base excision
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DNA replication
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transduction
Questão 83
Questão
__________ is initiated at sequences called origins
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DNA replication
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translation
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splicing
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transcription
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transposition
Questão 84
Questão
The process of __________ requires the activity of DNA ligase.
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translation
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capping
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transcription
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DNA replication
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transduction
Questão 85
Questão
RNA polymerase is primarily responsible for
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DNA replication
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translation
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transcription
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transformation
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polyadenylation
Questão 86
Questão
The process of __________ requires participation of tRNA molecules
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DNA replication
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capping
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transduction
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translation
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dark repair
Questão 87
Questão
The process of __________ is initiated with the incorporation of fMet.
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translation
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transcription
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capping
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DNA replication
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light repair
Questão 88
Questão
Initiation of __________ involves Sigma factor molecules.
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DNA replication
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translation
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transcription
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splicing
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termination
Questão 89
Questão
The process of __________ is described as semiconservative.
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translation
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transcription
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mismatch repair
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transformation
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DNA replication
Questão 90
Questão
During __________, the growing polymer is located in the P site.
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polyadenylation
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DNA replication
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mismatch repair
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transcription
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translation
Questão 91
Questão
Standard methods of sterilization are not effective in inactivating
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viruses
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bacterial cells
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prions
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bacterial endospores
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fungi
Questão 92
Questão
Which of the following statements is true of disinfectants?
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Disinfectants are effective in destroying endospores.
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Disinfectants are used on living tissue.
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Disinfectants are used for sterilization
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Disinfectants are used on inanimate surfaces.
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Disinfectants are only effective for short periods of time (seconds to minutes).
Questão 93
Questão
Which of the following statements concerning microbial death is FALSE?
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It can be used to evaluate the efficacy of antimicrobial agents.
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It is constant over time of exposure to an antimicrobial agent.
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It is the permanent loss of a microbe's reproductive ability.
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It is the permanent loss of a microbe's ability to reproduce and can be used to evaluate antimicrobial agents
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It is not an effective means of evaluating the efficacy of antimicrobial agents.
Questão 94
Questão
Which of the following is an example of sanitization?
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A public toilet is treated with disinfectants.
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A surgeon washes her hands before surgery
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Heat is used to kill potential pathogens in apple juice.
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An autoclave is used to prepare nutrient agar.
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A nurse prepares an injection site with an alcohol swab.
Questão 95
Responda
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sterile
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free of all microbes
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clean
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sanitized
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free of pathogens
Questão 96
Questão
Which of the following would NOT be bacteriostatic?
Questão 97
Questão
Antimicrobial agents that damage nucleic acids also affect
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the cell wall
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the cell membrane
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the viral envelope
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endospores
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protein synthesis
Questão 98
Questão
Seventy percent alcohol is effective against
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enveloped viruses
-
noneveloped viruses
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bacterial endospores
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protozoan cysts
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prions
Questão 99
Questão
An instrument that will come into contact with only the skin of a patient should be disinfected with a(n)
Questão 100
Questão
Which of the following is NOT an effective means of sterilization?
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-
ionizing radiation
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incineration
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lyophilization
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autoclaving
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dry heat
Questão 101
Questão
Which of the following describes flash pasteurization?
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heating at 63°C for 30 minutes
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heating at 72°C for 15 seconds
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heating at 72°C for 15 minutes
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heating at 134°C for one second
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passing liquid through steam at 140°C
Questão 102
Questão
The dairy creamer used in restaurants is usually sterilized by
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filtration
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ionizing radiation
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lyophilization
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UHT sterilization
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autoclaving
Questão 103
Questão
Boiling water for 10 minutes is effective in ridding it of
Questão 104
Questão
Which of the following is NOT a feature associated with filtration?
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-
nitrocellulose or plastic membrane filters
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sterilization of heat-sensitive materials
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varying thicknesses of membrane filters used
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use of HEPA filters to filter air
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ability of some filters to trap viruses and proteins
Questão 105
Questão
Which of the following is a target of pasteurization?
Questão 106
Questão
Which of the following types of radiation is nonionizing and has the shortest wavelength?
Responda
-
ultraviolet light
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infrared radiation
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microwaves
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X rays
-
gamma rays
Questão 107
Questão
Which of the following is used for microbial control in fresh fruits and vegetables?
Responda
-
X rays
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ultraviolet
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electron beams
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microwaves
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gamma rays
Questão 108
Questão
Which of the following can be used to disinfect air?
Questão 109
Questão
Lysol is an example of which of the following groups of chemical antimicrobial agents?
Responda
-
halogens
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phenolics
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alcohols
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aldehydes
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surfactants
Questão 110
Questão
Which of the following is the most appropriate pairing of microbe and biosafety level?
Questão 111
Questão
Betadine is an example of which of the following groups of antimicrobial agents?
Responda
-
alcohols
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heavy metals
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halogens
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phenolics
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surfactants
Questão 112
Questão
Which of the following statements about quaternary ammonium compounds is FALSE?
Responda
-
They are a type of detergent.
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Zephiran is an example of a quat.
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They are not effective against Pseudomonas aeruginosa.
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They function by cross-linking proteins.
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They are harmless to humans except at high concentrations.
Questão 113
Questão
Which of the following is a sterilizing agent?
Questão 114
Questão
Which of the following was used in the past to prevent the transmission of gonorrhea from an infected mother to her newborn?
Responda
-
hydrogen peroxide
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beta-propiolactone
-
thimerosal
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hexachlorophene
-
silver nitrate
Questão 115
Questão
Which of the following is used to sterilize items that should not, or cannot, be exposed to heat or water?
Responda
-
ethylene oxide
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formaldehyde
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hydrogen peroxide
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calcium hypochlorite
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triclosan
Questão 116
Questão
Which of the following statements about aldehydes is FALSE?
Responda
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They are used only to preserve dead tissues.
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They denature proteins.
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Some aldehydes can sterilize after long periods of exposure.
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They are usually hazardous to humans.
-
They are used in aqueous solutions.
Questão 117
Questão
Disinfecting agents naturally produced by microorganisms are
Responda
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aldehydes
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antimicrobials.
-
halogens
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quats
-
triclosans
Questão 118
Questão
A chemical agent that kills pathogenic microbes in general is a(n)
Responda
-
sanitizer
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germicide
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disinfectant
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fungicide
-
antiseptic
Questão 119
Questão
Which of the following is NOT a desirable characteristic of an ideal antimicrobial agent?
Questão 120
Questão
The endospores of which of the following microbes are used to measure the effectiveness of autoclave sterilization?
Questão 121
Questão
A scientist develops a new medication that is a protein compound and that must be administered by injection. Which of the following would be the most effective and safest means of preparing a sterile solution of the new medication?
Responda
-
autoclaving
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filtration
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dilution with alcohol
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lyophilization
-
ultraviolet irradiation
Questão 122
Questão
Which of the following procedures is currently the standard test used in the United States for evaluating the efficiency of antiseptics and disinfectants?
Responda
-
use-dilution test
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microbial death rate
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in-use test
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thermal death point
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phenol coefficient
Questão 123
Questão
Which of the following would be used to sterilize a mattress?
Responda
-
autoclaving
-
formaldehyde
-
ethylene oxide
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heavy metals
-
radiation
Questão 124
Questão
Disinfectants that damage membranes include
Questão 125
Questão
Which of the following antimicrobial agents is the most toxic to humans?
Responda
-
70 % alcohol
-
ethylene oxide
-
quats
-
chloramines
-
iodophors
Questão 126
Questão
The process of filtration is a(n)
Questão 127
Questão
Hydrogen peroxide is a(n)
Questão 128
Questão
The process of incineration is used for
Questão 129
Questão
Alcohols are used for
Questão 130
Questão
The chemical agents known as "quats" are used for
Questão 131
Questão
The compound ethylene oxide is used in
Responda
-
sanitization.
-
sterilization.
-
antisepsis.
-
degerming.
-
disinfection.
Questão 132
Questão
Glutaraldehyde is used for
Questão 133
Questão
__________ may be achieved using chlorine dioxide.
Questão 134
Questão
Gamma irradiation is a process for
Questão 135
Questão
__________ can be accomplished using boiling water.
Questão 136
Questão
Obligate anaerobes have enzymes such as superoxide dismutase to protect them from the damaging effects of oxygen.
Questão 137
Questão
Nitrogen fixation is a process that occurs in all bacteria.
Questão 138
Questão
Thermoduric mesophiles are often responsible for spoilage of improperly canned foods.
Questão 139
Questão
An obligate halophile will burst if placed in freshwater.
Questão 140
Questão
The only effective way to store bacterial cultures for short periods of time is to arrest their metabolism by freezing.
Questão 141
Questão
Quorum sensing is a process by which bacteria respond to the density of other bacteria in their environment.
Questão 142
Questão
An obligate anaerobe can be cultured in a candle jar.
Questão 143
Questão
Agar is a useful compound in the microbiology lab because it is an excellent nutrient for bacteria.
Questão 144
Questão
A selective medium can be formulated either by including inhibitory chemical substances or by leaving out a single crucial nutrient.
Questão 145
Questão
In spectrophotometry, 40% light transmission is the same thing as 60% absorbance of light.
Questão 146
Questão
Prokaryotic cells are diploid.
Questão 147
Questão
Prokaryotes have one type of DNA polymerase, which is different from that of eukaryotes.
Questão 148
Questão
The most common type of mutation is a point mutation.
Questão 149
Questão
Most bacteria have a natural ability to take up DNA from their environment.
Questão 150
Questão
The structure of DNA explains both its ability to encode genetic information and the way in which it is copied during cell reproduction.
Questão 151
Questão
DNA, which is negatively charged, wraps around positively charged histones as part of the packaging of eukaryotic chromosomes.
Questão 152
Questão
Bidirectional replication means that each strand of a DNA molecule is replicated in the opposite direction from the other.
Questão 153
Questão
The phenotype of an organism reflects only part of its genotype.
Questão 154
Questão
In generalized transduction, viruses carry random DNA sequences from one cell to another.
Questão 155
Questão
DNA polymerase III participates in the dark repair mechanism of DNA mutation repair.
Questão 156
Questão
An environment may contain some microbes and still be considered sterile.
Questão 157
Questão
Some viruses are inactivated by the same chemical or physical agents that damage cytoplasmic membranes.
Questão 158
Questão
UV light has the most effect on protein structure.
Questão 159
Questão
Antimicrobial agents usually work best at high temperatures and pH levels.
Questão 160
Questão
The phenol coefficient is one of the most widely used measurements of an antimicrobial agent's effectiveness.
Questão 161
Questão
No chemical or antimicrobial agents inactivate prions.
Questão 162
Questão
The decimal reduction time is the time required to kill all the microbes in a given sample.
Questão 163
Questão
Slow freezing is more damaging to microbial cells than quick freezing.
Questão 164
Questão
Hydrogen peroxide is an effective antiseptic.
Questão 165
Questão
By themselves, soaps have only degerming activity, not antimicrobial activity.