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Application of Molecular Biology to Medicine

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Quiz on Application of Molecular Biology to Medicine , created by MPusey on 05/01/2015.
MPusey
Quiz by MPusey, updated more than 1 year ago
MPusey
Created by MPusey over 9 years ago
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Resource summary

Question 1

Question
What type of chromosome does G-banding "line-up"?
Answer
  • Metaphase
  • Anaphase
  • Telophase
  • Prophase

Question 2

Question
What stain does G-banding use?
Answer
  • Giemsa
  • Chrome red
  • Methyl-blue
  • Green

Question 3

Question
G-banding can only detect large abnormalities. True or false?
Answer
  • True
  • False

Question 4

Question
What does FISH stand for?
Answer
  • Fluorescent in situ hybridization
  • Fluorescent in site hydrolysis
  • Familial in situ hybridization
  • Familial in situ hydrolysis

Question 5

Question
You don't need to know what abnormality you are looking for when using FISH. True or false?
Answer
  • True
  • False

Question 6

Question
Why do you need to know what abnormality you are looking for when using FISH?
Answer
  • Because it uses fluorescent probes to bind to specific sequences
  • Because it used restriction enzymes marked with fluorescent probes to cut DNA at a specific sequence

Question 7

Question
How can you tell if there is an abnormality using the FISH technique?
Answer
  • Because some of the markers will not light up as they have not been able to anneal to bits of DNA sequence as they are missing
  • Because all of the markers will light up because there are specific sequences of DNA that cause genetic disorders
  • Because if there were no abnormalities the markers would not show up at all

Question 8

Question
FISH can show slightly smaller abnormalities than G-banding, but they still have to fairly large. True or false?
Answer
  • True
  • False

Question 9

Question
What does QF-PCR stand for?
Answer
  • Quantitative fluorescence Polymerase Chain Reaction
  • Qualitative fluorescence polymerase chain reaction
  • Quantitative familial polymerase chain reaction
  • Qualitative familial polymerase chain reaction

Question 10

Question
What is QF-PCR used to detect?
Answer
  • Trisomy syndromes
  • Abnormalities in chromosome size
  • Deletion syndromes
  • Abnormalities in chromosome banding

Question 11

Question
What is the name for trisomy 13?
Answer
  • Pattau's syndrome
  • Edward's syndrome
  • Down's syndrome

Question 12

Question
What is the name for trisomy 18?
Answer
  • Pattau's syndrome
  • Edward's syndrome
  • Down's syndrome

Question 13

Question
What is the name for trisomy 21?
Answer
  • Pattau's syndrome
  • Edward's syndrome
  • Down's syndrome

Question 14

Question
Which biological technique is quickest?
Answer
  • FISH
  • G-banding
  • QF-PCR

Question 15

Question
You have to know what abnormality you are looking for when using QF-PCR. True or false?
Answer
  • True
  • False

Question 16

Question
What sort of abnormalities does array-CGH detect?
Answer
  • Larger abnormalities
  • Smaller abnormalities

Question 17

Question
What is DNA labelled with in array-CGH?
Answer
  • Fluorescent dye
  • Coloured markers
  • Radiation

Question 18

Question
What does the CGH in array-CGH stand for?
Answer
  • Comparative genomic hybridization
  • Comparative genomic hydrolysis
  • Comparative genetic hybridization
  • Comparative genetic hydrolysis

Question 19

Question
How can you tell if there is an abnormality in the DNA when using array-CGH?
Answer
  • The relative fluorescence of the sample and control strand will be different
  • The relative radiation of the sample and control strand will be different
  • The length of the sample and control strand will be different when run through gel electrophoresis

Question 20

Question
Which two molecular biological techniques look at the actual base sequence when looking for genetic abnormalities?
Answer
  • G-banding
  • FISH
  • QF-PCR
  • Array-CGH
  • Sanger sequencing
  • Next generation sequencing

Question 21

Question
Which of the following is cheaper and less time consuming?
Answer
  • Sanger sequencing
  • Next generation sequencing

Question 22

Question
Why do we only sequence exons when using sanger sequencing?
Answer
  • Because the introns do not code for proteins so are not important to us
  • Because it is time consuming and expensive
  • Because introns rarely have mutations that cause genetic diseases
  • Because we are unable to isolate the introns to sequence them

Question 23

Question
What machine is used in sanger sequencing to read the sequence of bases?
Answer
  • An electropheragram
  • A Geiger counter
  • A microscope

Question 24

Question
Not all changes to the base sequence cause disease. True or false?
Answer
  • True
  • False

Question 25

Question
Why is it better to use next generation sequencing when you don't know what you are looking for?
Answer
  • Because it is easy to sequence a whole genome
  • Because sanger sequencing would take too long
  • Because next generation sequencing is more accurate
  • Because next generation sequencing is less expensive
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